Protocol for producing brain-derived neurotrophic factor and neurotrophin-4 in their pro and active form in Escherichia coli

被引：0

作者：

Holzner, Christoph ^{[1
,2
]}

Boettinger, Katharina ^{[1
,2
]}

Huber, Christian G. ^{[1
,2
]}

Dall, Elfriede ^{[1
,2
]}

Brandstetter, Hans ^{[1
,2
]}

机构：

[1] Univ Salzburg, Dept Biosci & Med Biol, Hellbrunner Str 34, A-5020 Salzburg, Austria

[2] Univ Salzburg, Ctr Tumor Biol & Immunol CTBI, A-5020 Salzburg, Austria

来源：

STAR PROTOCOLS | 2025年 / 6卷 / 02期

基金：

奥地利科学基金会;

关键词：

biotechnology and bioengineering; protein biochemistry; protein expression and purification;

D O I：

10.1016/j.xpro.2025.103715

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT4) are neurotrophic growth factors, signaling primarily via the tropomyosin receptor kinase B (TrkB). Here, we present a protocol for producing BDNF and NT4 in their pro and active form in Escherichia coli. We describe steps for E. coli expression, oxidative in vitro folding from inclusion bodies, and purification. We then detail procedures for proteolytic activation and characterization of the purified neurotrophins. For complete details on the use and execution of this protocol, please refer to Holzner et al.1

引用

页数：18