Mussel-inspired surface-engineering of 3D printed scaffolds employing bedecked transition metal for accelerated bone tissue regeneration

In modern civilization with fast work culture and uncontrolled lifestyles increase bone-related problems, moreover, lack of auto-regeneration of bone, indulge to formulate a suitable methodology to get rid of this problem. In this article, nanohydroxyapatite (nHA) decorated hierarchical titanium phosphate (TP) was synthesized by solvothermal process and incorporated into newly synthesized tartaric acid-based polyurethane (PU) through in situ technique. The porous 3D scaffold was fabricated by most advanced 3D printing technique with desired porous structure in a controlled manner. The biochemical properties of scaffold's surface were improved via immobilizing polydopamine (PDA) at ambient temperature. Elemental analysis indicated that TP-doped nanohybrid scaffolds experienced higher amount of PDA immobilization as compared to pristine and nHAdoped scaffolds. The unoccupied 'd' orbital of introduced Ti can form a coordinate bond with catechol groups of dopamine (DA) which augments PDA deposition on the scaffold's surface. Furthermore, the higher effective nuclear charge (Z*) of tetravalent Ti ion generates an effective dative bond with the urethane groups of PU chain which improves hardness and tensile strength (TS) of produced nanocomposites (PU/TP-nHA) remarkably by 71.3 % and 126 % compared to pristine PU. Ti-doped nanohybrid scaffolds, containing calcium and phosphate components with higher amounts of deposited PDA exhibited improved in vitro osteogenic bioactivity. Moreover, in vivo study expressed superior bone regeneration efficacy of the TP-doped nHA-integrated PU scaffold without showing any organ toxicity. Thus, the optimum level of TP-doped nHA with higher amount of PDAimmobilized PU nanohybrid scaffold would be a suitable bone graft substitute in bone regeneration applications.