Fertility programs for lactating dairy cows: A novel presynch plus timed artificial insemination program (Double E-Synch) produces similar ovarian dynamics, synchronization, and fertility as Double-Ovsynch

被引:1
作者
Consentini, Carlos E. C. [1 ,2 ,3 ]
Abadia, Tattiany [4 ]
Galindez, Juan P. A. [1 ]
Lopes, Ana L. M. [1 ]
Ferro, Pedro P. C. [1 ]
Pazini, Yasmim E. [1 ]
Faria, Natalia, V [1 ]
Machado, Fernando [4 ]
Capella, Tuanne [4 ]
dos Santos, Tiago N. [4 ]
Duarte, Marcelo [4 ]
Ferreira, Paulo P. [4 ]
Matos, Luiz M. F. [4 ]
Ferreira, Danilo R. [4 ]
Campos, Ernane [5 ]
Prata, Alexandre [3 ]
Melo, Leonardo F. [6 ]
Wiltbank, Milo C. [2 ]
Sartori, Roberto [1 ]
机构
[1] Univ Sao Paulo, Dept Anim Sci, BR-13418900 Piracicaba, SP, Brazil
[2] Univ Wisconsin, Dept Anim & Dairy Sci, Madison, WI 53706 USA
[3] GlobalGen Vet Sci, BR-14887360 Jaboticabal, SP, Brazil
[4] Ceu Azul Farm, BR-75180000 Silvania, GO, Brazil
[5] Rehagro, BR-37200000 Lavras, MG, Brazil
[6] Univ Fed Goias, Sch Vet & Anim Sci, BR-74690900 Goiania, GO, Brazil
基金
巴西圣保罗研究基金会;
关键词
dairy cow; timed artificial insemination; fertility; synchronization; GONADOTROPIN-RELEASING-HORMONE; BODY CONDITION SCORE; PREGNANCY RATES; MILK-PRODUCTION; AI PROTOCOLS; PROGESTERONE SUPPLEMENTATION; REPRODUCTIVE MANAGEMENT; CHORIONIC-GONADOTROPIN; ESTRADIOL CYPIONATE; OVULATORY FOLLICLE;
D O I
10.3168/jds.2024-25221
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Fertility programs were implemented for the first postpartum timed artificial insemination (TAI) in 800 (primiparous and multiparous) lactating dairy cows, evaluating 2 presynchronization (presynch) strategies and 2 TAI protocols, in a 2 x 2 factorial arrangement. Weekly, cows were enrolled into 1 of 4 groups (Ovs+Ovs [Double-Ovsynch], Ovs+OvsP4/E2, PreP4/E2+Ovs, and PreP4/E2+OvsP4/E2 [Double E-Synch]). On d -17 (34 +/- 3 DIM), the Ovsynch [Ovs] presynch was initiated with 10 mu g of buserelin acetate (GnRH), and cows received 0.5 mg of cloprostenol sodium PGF2 alpha analog (PGF) on d -10, and 10 mu g of GnRH on d -7. The PreP4/E2 presynch was initiated on d -17 with a used 2-g progesterone (P4) insert, which was removed on d -10, together with 0.5 mg of PGF and 1 mg of estradiol (E2) cypionate (EC). For TAI protocols, Ovs group received the following: on d 0, 20 mu g of GnRH (double dose); on d 7, PGF; on d 8, PGF; on d 9.5, 10 mu g of GnRH; and on d 10, TAI (16 h after GnRH). Cows submitted to OvsP4/E2 received the following: on d 0, 20 mu g of GnRH (double dose) and a new 2-g P4 insert; on d 7, PGF; on d 8, P4 insert removal, PGF, and EC; and on d 10, TAI (48 h after P4 insert removal). The GLIMMIX procedure of SAS 9.4 was used for statistical analyses (P <= 0.05). The presence of corpus luteum (CL) on d -17 (average = 68.8% [550/800]) was similar among treatments. The presence of CL on d 0 of TAI protocols was high, and Ovs as a presynch increased percentage of cows with CL (95.5% [382/400] vs. 90.8% [363/400]). However, at the first PGF of the breeding (TAI) protocols (d 7), there was no effect of presynchronization program and 98.5% (788/800) of the cows had at least 1 CL. Ovulation after d 0 was greater in cows submitted to PreP4/E2 than Ovs (72.0% [288/400] vs. 64.3% [257/400]), and those ovulating had greater pregnancies per artificial insemination (P/AI; 51.0% [278/545] vs. 41.6% [106/255]). Overall, multiple ovulations after TAI were low and similar between TAI protocols and presynch strategies (7.2% [54/753]). Expression of estrus in OvsP4/E2 protocols was greater than Ovs (69.4% [274/395] vs. 41.5% [168/405]), and an interaction was detected, in which cows not expressing estrus ovulated more after TAI in Ovs compared with OvsP4/E2 protocol (93.3 [221/237] vs. 77.7% [94/121]). Cows expressing estrus had greater P/AI in both Ovs (58.3 [98/168] vs. 42.2% [100/237]) and OvsP4/E2 (57.3 [157/274] vs. 24.0% [29/121]). There was no interaction between presynch and TAI protocol on P/AI on d 32 of cows that ovulated after TAI (48.4%, 49.7%, 53.3%, and 52.5% for Ovs+Ovs, Ovs+OvsP4/E2, PreP4/E2+Ovs, and PreP4/E2+OvsP4/E2, respectively), and no differences in pregnancy loss between d 32 and 90 (average = 24.0% [92/384]). In conclusion, the study validated 2 presynchronization strategies and 2 TAI protocols, establishing 4 possible fertility programs, all of them producing well-controlled ovarian dynamics, excellent synchronization, and high fertility. Moreover, DoubleOvsynch and Double E-Synch both produced similar results, despite differences in pharmacological bases.
引用
收藏
页码:4435 / 4447
页数:13
相关论文
共 64 条
[1]  
[Anonymous], 2001, Nutrient Requirements of Dairy Cattle, V7th revis, DOI DOI 10.17226/9825
[2]   Double-Ovsynch in high-producing dairy cows: Effects on progesterone concentrations and ovulation to GnRH treatments [J].
Ayres, H. ;
Ferreira, R. M. ;
Cunha, A. P. ;
Araujo, R. R. ;
Wiltbank, M. C. .
THERIOGENOLOGY, 2013, 79 (01) :159-164
[3]   Association of changes among body condition score during the transition period with NEFA and BHBA concentrations, milk production, fertility, and health of Holstein cows [J].
Barletta, R. V. ;
Maturana Filho, M. ;
Carvalho, P. D. ;
Del Valle, T. A. ;
Netto, A. S. ;
Renno, F. P. ;
Mingoti, R. D. ;
Gandra, J. R. ;
Mourao, G. B. ;
Fricke, P. M. ;
Sartori, R. ;
Madureira, E. H. ;
Wiltbank, M. C. .
THERIOGENOLOGY, 2017, 104 :30-36
[4]   Optimizing ovulation to first GnRH improved outcomes to each hormonal injection of Ovsynch in lactating dairy cows [J].
Bello, N. M. ;
Steibel, J. P. ;
Pursley, J. R. .
JOURNAL OF DAIRY SCIENCE, 2006, 89 (09) :3413-3424
[5]   Progesterone supplementation to lactating dairy cows without a corpus luteum at initiation of the Ovsynch protocol [J].
Bisinotto, R. S. ;
Castro, L. O. ;
Pansani, M. B. ;
Narciso, C. D. ;
Martinez, N. ;
Sinedino, L. D. P. ;
Pinto, T. L. C. ;
Van de Burgwal, N. S. ;
Bosman, H. M. ;
Surjus, R. S. ;
Thatcher, W. W. ;
Santos, J. E. P. .
JOURNAL OF DAIRY SCIENCE, 2015, 98 (04) :2515-2528
[6]   Meta-analysis of progesterone supplementation during timed artificial insemination programs in dairy cows [J].
Bisinotto, R. S. ;
Lean, I. J. ;
Thatcher, W. W. ;
Santos, J. E. P. .
JOURNAL OF DAIRY SCIENCE, 2015, 98 (04) :2472-2487
[7]   Luteal Presence and Ovarian Response at the Beginning of a Timed Artificial Insemination Protocol for Lactating Dairy Cows Affect Fertility: A Meta-Analysis [J].
Borchardt, Stefan ;
Pohl, Alina ;
Heuwieser, Wolfgang .
ANIMALS, 2020, 10 (09) :1-15
[8]   Effects of additional prostaglandin F2α and estradiol-17β during Ovsynch in lactating dairy cows [J].
Brusveen, D. J. ;
Souza, A. H. ;
Wiltbank, M. C. .
JOURNAL OF DAIRY SCIENCE, 2009, 92 (04) :1412-1422
[9]   Economics of Twin Pregnancies in Dairy Cattle [J].
Cabrera, Victor E. ;
Fricke, Paul M. .
ANIMALS, 2021, 11 (02) :1-14
[10]   Factors That Optimize Reproductive Efficiency in Dairy Herds with an Emphasis on Timed Artificial Insemination Programs [J].
Cardoso Consentini, Carlos Eduardo ;
Wiltbank, Milo Charles ;
Sartori, Roberto .
ANIMALS, 2021, 11 (02) :1-30