A novel and universal dual-functionalized Hazo-POPs@COOH-apt/ PGE-based electrochemical biosensor for detection of aflatoxin M1 (AFM1) in raw milk sample: A versatile peroxidase-mimicking aptananozyme approach

被引：0

作者：

Huma, Zill-E ^{[1
,3
]}

Nazli, Zill-I-Huma ^{[1
]}

Gokce, Gultekin ^{[2
]}

Ali, Mustafa ^{[3
]}

Jubeen, Farhat ^{[1
]}

Hayat, Akhtar ^{[3
]}

机构：

[1] Govt Coll Women Univ, Dept Chem, Arfa Kareem Rd, Faisalabad 38000, Pakistan

[2] Sivas Cumhuriyet Univ, Dept Analyt Chem, Sivas, Turkiye

[3] COMSATS Univ Islamabad, Interdisciplinary Res Ctr Biomed Mat IRCBM, Lahore Campus,1-5 km Def Rd,Raiwind Rd, Lahore 54000, Punjab, Pakistan

来源：

MATERIALS CHEMISTRY AND PHYSICS | 2025年 / 341卷

关键词：

Biosensor; Nanozyme; Aptananozyme; O-hydroxyazobenzene polymers; Peroxidase-like activity; AFM1; APTAMER; NANOPARTICLES; APTASENSOR; FABRICATION; OCHRATOXIN; NANOZYMES; DESIGN; LABEL; FILM;

D O I：

10.1016/j.matchemphys.2025.130887

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A dual-functionalized electrochemical biosensor was developed for ultrasensitive detection of aflatoxin M1 (AFM1) in milk. AFM1, a hydroxylated derivative of aflatoxin B1 (AFB1), poses significant health risks, including reduced liver function, stunted growth in children, and immunosuppression. With no FDA-approved decontamination methods, a point-of-care (POC) device for rapid and precise AFM1 detection is crucial. The electrochemical biosensor combines specificity from COOH-functionalized AFM1-Aptamer (COOH-Apt) and ohydroxyazobenzene polymers (Hazo-POPs) as a nanozyme probe at pencil graphite electrode (PGE). Hazo-POPs exhibiting integrated electroactive potential and peroxidase-like activity, independently utilized for AFM1 detection. Hazo-POPs were characterized using XRD, FTIR, UV-Vis, DLS, Raman spectroscopy, and FESEM and BET. At the same time, PGE surface modification was analyzed through optical microscopy, contact angle measurement, FESEM, micro-CT, CV, and EIS. In method, I, PGE was modified with Hazo-POPs@COOH-Apt for an optimized electrochemical response. COOH-Apt immobilization (Hazo-POPs@COOH-Apt/PGE) exhibited increased current, whereas AFM1 incubation (Hazo-POPs@COOH-Apt/PGE/AFM1) blocked the electron transfer, leading to decreased current. Method II employed Hazo-POPs' peroxidase-like activity, measuring the DPV response of 3,3 ',5,5 '-tetramethylbenzidine (TMB), confirming aptananozyme (Hazo-POPs@COOH-Apt/PGE) specificity for AFM1. H2O2 generated hydroxyl radicals (degrees OH) in the presence of Hazo-POPs and TMB was oxidized to diimine by these radicals, leading to reduced TMB peak values, indicating oxidation. The electrochemical biosensor (Hazo-POPs@COOH-Apt/PGE) exhibited a broad linear range (0.005-500 nM) and low LOD (0.004 nM in method I; 0.003 nM in method II). Validated in spiked milk samples, it showed high recovery (101.21-104 %), exceptional sensitivity, reproducibility, and stability for three weeks.

引用

页数：10