This study aims to explore the relationship between the provision of ecosystem services (ESs) and other territorial characteristics. Taking Italian Metropolitan Regions (MRs) as case studies, the gradient of specialization providing a set of ESs in different territorial contexts is examined using the National Strategy for Internal Areas (SNAI) territorial classification. The main objective of this research is to understand whether there is a spatial pattern of location of different ESs within metropolitan SNAI areas. Inspired by Von Th & uuml;nen's spatial economic theories, this study explores how proximity to urban centers influences land use and ES specialization. Through land use analysis and the calculation of a SI, we evaluate patterns in ES supply, based on a benefit transfer approach. The results show that the MRs provide about EUR 14.6 billion per year in benefits, equivalent to 15% of the national wealth in environmental goods and services. At the SNAI area scale, internal areas have the highest average economic values per hectare, while the central areas have lower economic values. This trend is confirmed by the calculation of the specialization index (SI) in line with Von Th & uuml;nen's theorem as follows: central areas are specialized in the provision of bundles of ESs related to intensive land use (e.g., food production), while the peripheral areas are specialized in the supply of regulation ESs related to more natural areas. The findings underline significant policy implications for metropolitan planning, stressing the need for the balanced management of ESs to address urban demands and enhance resilience. This research contributes to understanding the spatial dynamics of ES supply, offering a basis for tailored interventions in metropolitan and national contexts.