Cholesterol Affects the Pore Formation and the Membrane–Membrane Interaction Induced by an Antimicrobial Peptide, NK-2, in Phospholipid Vesicles

被引：0

作者：

Surajit Das ^{[1
]}

Rajeev Jain ^{[2
]}

Kalyan Kumar Banerjee ^{[3
]}

Pabitra Maity ^{[1
]}

Krishnananda Chattopadhyay ^{[1
]}

Sanat Karmakar ^{[2
]}

机构：

[1] Soft Matter and Biophysics Laboratory, Department of Physics, Jadavpur University, 188, Raja S. C. Mallick Road, Kolkata

[2] Structural Biology & Bio-Informatics Division, CSIR, Indian Institute of Chemical Biology, Kolkata

[3] Academy of Scientific and Innovative Research (AcSIR), CSIR- Human Resource Development Centre Campus, Uttar Pradesh, Ghaziabad

来源：

The Journal of Membrane Biology | 2025年 / 258卷 / 3期

关键词：

Antimicrobial peptide; Cholesterol; Membrane; Phase contrast microscopy; Vesicles;

D O I：

10.1007/s00232-025-00347-2

中图分类号：

学科分类号：

摘要：

Antimicrobial peptides are part of the innate immune response and show their antimicrobial activity by forming pores, followed by disintegration of the membrane. Cholesterol in the membrane can affect the pore formation process, as cholesterol is known to alter the permeability and elastic properties of the membrane. The present research systematically explores the role of cholesterol in modulating the interaction of the antimicrobial peptide NK-2 with phospholipid membranes, as well as the processes of pore formation induced by NK-2 within the membrane. Large unilamellar vesicles (LUVs) and giant unilamellar vesicles (GUVs) made from DOPC-DOPG and Egg PC with varying cholesterol concentrations have been studied using a variety of experimental techniques. The present study revealed that both the magnitude of zeta potential and surface charge density diminished as cholesterol concentrations increased at an intermediate NK-2 concentration. The proliferation of the size distributions of LUVs containing cholesterol when exposed to NK-2 indicates the occurrence of vesicle aggregation. The phase contrast micrographs of GUVs as well as the calcein release experiments on LUVs show evidence of pores. Notably, the incorporation of cholesterol into the membrane was found to have a significant effect on both the permeability of the membrane and the kinetics of the pore formation process. This biophysical research contributes essential knowledge regarding the role of cholesterol in influencing the antimicrobial efficacy of the membrane. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2025.

引用

页码：237 / 252

页数：15

共 47 条

[1]

Alam J.M., Kobayashi T., Yamazaki M., The single-giant unilamellar vesicle method reveals LyseninInduced pore formation in lipid membranes containing sphingomyelin, Biochemistry, 51, pp. 5160-5172, (2012)

[2]

Allende D., Simon S.A., Mcintosh T.J., Melittin-induced bilayer leakage depends on lipid material properties: Evidence for toroidal pores, Biophys J, 88, pp. 1828-1837, (2005)

[3]

Angelova M.I., Dimitrov D.S., Liposome electroformation, Faraday Discuss Chem Soc, 81, pp. 303-311, (1986)

[4]

Banerjee K.K., Maity P., Das S., Karmakar S., Effect of cholesterol on the ion-membrane interaction: Zeta potential and dynamic light scattering study, Chem Phys Lipids, 254, (2023)

[5]

Bechinger B., Structure and functions of channel-forming peptides: magainins, cecropins, melittin and alamethicin, J Membr Biol, 156, pp. 197-211, (1997)

[6]

Benfield A.H., Henriques S.T., Mode-of-action of antimicrobial peptides: Membrane disruption vs intracellular mechanisms, Front Med Technol, 2, (2020)

[7]

Brender J.R., McHenry A.J., Ramamoorthy A., Does cholesterol play a role in the bacterial selectivity of antimicrobial peptides?, Front Immunol, 3, (2012)

[8]

Brogden K.A., Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?, Nat Rev Microbiol, 3, pp. 238-250, (2005)

[9]

Cascales J.J.L., Zenak S., de Torre J.G.L., Lezama O.G., Garro A., Enriz R.D., Small cationic peptides: Influence of charge on their antimicrobial activity activity, ACS Omega, 3, pp. 5390-5398, (2018)

[10]

Chattopadhyay A., Raghuraman H., Interaction of Melittin with membrane cholesterol: A fluorescence approach, Biophys J, 87, pp. 2419-2432, (2004)

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