Extraction, structural characterization and biological activities of exopolysaccharides from Ruditapes philippinarum

被引:0
作者
SHAN, Hongjie [1 ,2 ]
CHEN, Guoqiang [2 ,3 ,4 ]
DAI, Wenxue [2 ]
CHEN, Xuedong [2 ,3 ,4 ]
DONG, Sheng [2 ,3 ,4 ]
WEI, Yuxi [1 ]
ZHANG, Haibo [2 ,3 ,4 ]
机构
[1] College of Life Science, Qingdao University, Qingdao
[2] Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences, Qingdao
[3] Shandong Energy Institute, Qingdao
[4] Qingdao New Energy Shandong Laboratory, Qingdao
来源
Chinese Journal of Analytical Chemistry | 2025年 / 53卷 / 06期
基金
中国国家自然科学基金;
关键词
Antioxidant activity; Antitumor activity; Clam meat; Exopolysaccharides; Structural characterization;
D O I
10.1016/j.cjac.2025.100508
中图分类号
学科分类号
摘要
This study investigated the extraction, structural characterization and biological activities of exopolysaccharides from clam meat. The aim of this is to develop low-cost and novel animal polysaccharides with potential medicinal or health benefits. A crude polysaccharide yield of 10 g/100 g was extracted by the ultrasonic-assisted method. Two fraction exopolysaccharides (RPP-1A and RPP-2A) were obtained through DEAE-52 column chromatography and HW-65F column. Glc, GalA, GlcA, Rha and Rib were the main monosaccharides components of RPP-1A, whereas RPP-2A was primarily composed of GlcA, Rha and Rib. RPP-1A and RPP-2A were further investigated using Fourier transformed infrared (FT-IR) and nuclear magnetic resonance (NMR) that the results showed that RPP-1A comprised a main chain of residues represented as: [→6-α-Glc-(1 → 4)-β-GalA-(1 → 6)-α-Glc-(1 → 4)-β-GalA-(1→]. The side chain repeating unit structure is [β-GlcA-(1 → 4)-α-Glc-(1 → 3)-α-Rib-(1 → 4)-α-Rha-(1→], attached to the main chain at the C-2 position of Glc. RPP-2A represents the side chain portion of RPP-1A [β-GlcA-(1 → 4)-α-Glc-(1 → 3)-α-Rib-(1 → 4)-α-Rha-(1→]. Scanning electron microscopy (SEM) analysis revealed that the characteristic morphology of different fractions. X-ray diffraction showed that the polysaccharides consisted of crystalline and amorphous regions. Furthermore, assays of antioxidant activity showed that any one of RPP-1A and RPP-2A had antioxidant effects against DPPH radical, ABTS radical cation, hydroxyl radical, among which RPP-2 was stronger. In addition, they significantly inhibited the proliferation of Hela, and HepG2 cancer cells, and HepG2 was more sensitive to RPP-2A. In general, the results demonstrated that RPPs had great potential as a natural antioxidant in the functional food, and they are promising candidates for cancer treatment. © 2025
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