Novel CRISPR/Cas9-Based Approaches for Quantitative Study of DSB Repair Mechanics

被引：1

作者：

Smirnov, Alexander V. ^{[1
]}

Yunusova, Anastasia M. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Cytol & Genet, Siberian Branch, Novosibirsk 630090, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2025年 / 90卷 / 04期

关键词：

CRISPR/Cas9; double-strand break (DSB) repair; genetic reporter; DOUBLE-STRAND BREAKS; HOMOLOGOUS RECOMBINATION; HUMAN-CELLS; CRISPR DELETION; CAS9; DYNAMICS; TRANSLOCATIONS; INTERROGATION; NUCLEASES; INSIGHTS;

D O I：

10.1134/S0006297924601813

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This review examines modern approaches to studying double-strand break (DSB) DNA repair in mammalian cells, employing the CRISPR/Cas9 system. Due to its flexibility and efficacy, the Cas9 nuclease is used in numerous genetic reporters. We discuss various fluorescence-based genetic reporters used to monitor the repair process. In addition, among the innovative Cas9-based methods, special attention is given to the techniques that examine both single and multiple DSBs, including approaches such as DSB-TRIP and ddXR. These methods open new possibilities for investigating structural rearrangements or analyzing random genomic sites. Additionally, the review considers how DSBs induced by Cas9 differ from those made by other nucleases and how these peculiarities could impact DNA repair mechanisms. Understanding these differences is crucial for planning experiments aimed at studying DSB repair.

引用

页码：437 / 456

页数：20

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