The functionalized Ag@SiO2 core-shell composite for excellent enzyme matrix

被引：0

作者：

Ismillayli, Nurul ^{[1
]}

Suprapto, Suprapto ^{[2
]}

Jovita, Stella ^{[2
]}

Bahruji, Hasliza ^{[3
]}

Jalil, Aishah Abdul ^{[4
,5
]}

Hermanto, Dhony ^{[1
]}

Prasetyoko, Didik ^{[2
]}

机构：

[1] Univ Mataram, Fac Math & Nat Sci, Dept Chem, Mataram 83125, Indonesia

[2] Inst Teknol Sepuluh Nopember, Fac Sci & Data Analyt, Dept Chem, Surabaya 60111, Indonesia

[3] Univ Brunei Darussalam, Ctr Adv Mat & Energy Sci, Jalan Tungku Link, BE-1410 Gadong, Brunei

[4] Univ Teknol Malaysia, Inst Future Energy, Ctr Hydrogen Energy, Johor Baharu 81310, Johor, Malaysia

[5] Univ Teknol Malaysia, Fac Chem & Energy Engn, Dept Chem Engn, Johor Baharu 81310, Johor, Malaysia

来源：

JOURNAL OF MOLECULAR STRUCTURE | 2025年 / 1338卷

关键词：

Ag@SiO2; Core-shell; Composite; Enzyme matrix; IMMOBILIZATION; NANOPARTICLES; PARTICLES; IMPROVEMENT;

D O I：

10.1016/j.molstruc.2025.142308

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Enzyme immobilization in core-shell composite improves activity, maintains stability and allows reusability via facile separation. Alcohol oxidase (AOX) enzyme was immobilized on SiO2 nanoparticles (M1), Ag@SiO2 core-shell (M2), and NH2 functionalized Ag@SiO2 core-shell (M3) to improve activity at different pH and temperatures, and stability under long storage. Ag@SiO2 core-shell composite was produced using controlled condensation of Si precursor (TEOS) on Ag-stabilized PVP nanoparticles, followed by NH2-functionalization with APTES. A monodispersed core-shell composites with diameters of 94.71 +/- 6.16 nm were obtained with Ag fcc faced structure as core. NH2 functionalization on Ag@SiO2 (M3) formed covalent interactions with the immobilized AOX enzyme, preventing AOX from leaching. The immobilized AOX on NH2-functionalized Ag@SiO2 exhibits higher activity for alcohol oxidation than M2 and M1 matrices. The conductive nature of Ag in the composite core allows fast electron transfer between AOX and the substrate, enhancing enzyme stability when used in conditions other than their optimum.

引用

页数：8

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