CRISPR/Cas and Argonaute-based biosensors for nucleic acid detection

被引：0
作者：
Xiong, Qiangyuan [1 ,2 ]
Zhu, Cancan [1 ]
Yin, Xueer [1 ,2 ]
Zhu, Ling [1 ,2 ]
机构：
[1] Chinese Acad Sci, Anhui Inst Opt & Fine Mech, Hefei Inst Phys Sci, Hefei 230031, Peoples R China
[2] Univ Sci & Technol China, Hefei 230026, Peoples R China
来源：
TALANTA | 2025年 / 294卷
关键词：
CRISPR/Cas; Argonaute; Biosensor; Cascade amplification; Nucleic acid detection; AMPLIFICATION TECHNOLOGIES; RNA; DIAGNOSTICS; LIMITATIONS; MECHANISM; PROTEINS; SYSTEMS;
D O I：
10.1016/j.talanta.2025.128210
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Nowadays, nucleic acid detection technology has been applied to disease diagnosis, prevention, food safety, environmental testing and many other aspects. However, traditional methods still have shortcomings. Therefore, there is an urgent need for a simple, rapid, sensitive, and specific new method to supersede traditional nucleic acid detection technology. CRISPR/Cas(Clustered Regularly Interspaced Short Palindromic Repeats/CRISPRassociated) system and Argonaute (Ago) system play an important role in microbial immune defense. Their targeting specificity, programmability and special trans-cleavage activity make it possible to develop some new platforms for nucleic acid detection in combination with a variety of biosensors. We introduce the origins of these two systems and the biosensors developed based on CRISPR/Cas system and Ago system, respectively, especially the prospects for the future development of Cascade Amplification biosensors. This review is expected to provide useful guidance for researchers in related fields and provide inspiration for the development of Cascade Amplification biosensors in the future.
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页数：15
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