Inoculation with Pseudomonas Thivervalensis and Variovorax Paradoxus Enhances Yield and Grain Quality and Alters Rhizosphere Microbiota in Cereal-Legume Intercropping System

被引:0
作者
Ferchichi, Nouha [1 ,2 ]
Toukabri, Wael [1 ]
Barbouchi, Meriem [1 ]
Di Cesare, Francesca [3 ]
Bahri, Haithem [1 ]
M'hamed, Hatem Cheikh [1 ]
Hlel, Dorsaf [4 ]
Mengoni, Alessio [3 ]
Bacci, Giovanni [3 ]
Mhamdi, Ridha [2 ]
Annabi, Mohamed [1 ]
Trabelsi, Darine [2 ]
机构
[1] Carthage Univ, Natl Inst Agr Res Tunisia INRAT, Agron Sci & Tech Lab LR16 INRAT 05, Ariana 2049, Tunisia
[2] Ctr Biotechnol Borj Cedria CBBC, Lab Legumes & Sustainable Agrosyst, BP 901, Hammam Lif 2050, Ben Arous, Tunisia
[3] Univ Florence, Dept Biol, Florence, Italy
[4] Natl Inst Field Crops INGC, Bou Salem 8170, Jendouba, Tunisia
关键词
Intercropping; Soil rhizosphere; Bacterial microbiota; Legumes; Cereal; PGPR; PHOSPHORUS AVAILABILITY; SOIL; INCREASE; WHEAT;
D O I
10.1007/s42729-025-02454-4
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
PurposeCereal-legume intercropping and inoculation with Plant Growth-Promoting Rhizobacteria (PGPR) have been extensively documented to impact crop performance and the rhizosphere microbiome. However, information on their combined effects is limited, and the influence of PGPR-inoculation of legumes on the rhizosphere microbiome of neighboring cereals remains unknown. This study examines how legume intercropping with or without PGPR inoculation affects yield, grain quality, and rhizosphere bacterial diversity in durum wheat and barley.MethodsField experiments were conducted using three cropping systems: cereal sole cropping (SC), intercropping with legumes (IC), and intercropping with PGPR-inoculated legumes (ICC), where legumes included fenugreek and clover inoculated with a PGPR consortium (Pseudomonas thivervalensis and Variovorax paradoxus).ResultsThe IC system increased grain yield by 26% for durum wheat and 30% for barley compared to SC. The ICC system further enhanced yields by 34% and 41% for durum wheat and barley, respectively. High-throughput 16S rRNA sequencing revealed distinct changes in the rhizosphere bacterial communities in the ICC system, with 445 and 389 Amplicon Sequence Variants (ASVs) enriched compared to IC and SC, respectively. Dominant genera in ICC soils included Stenotrophomonas, Streptomyces, Pseudomonas, and Variovorax. Many of these taxa positively correlated with yield and quality parameters, with Streptomyces and Variovorax linked to increased yields, while Pseudomonas and Serratia were associated with soil health.ConclusionsThese findings demonstrate that cereal-legume intercropping, enhanced by PGPR inoculation, can significantly improve cereal yield and grain quality while enriching beneficial rhizosphere bacterial communities, highlighting a promising agroecological approach for sustainable agriculture.
引用
收藏
页码:5157 / 5172
页数:16
相关论文
共 66 条
[1]  
Anderson M.J., 2008, PERMANOVA+ for PRIMER: Guide to Software and Statistical Methods, P214
[2]  
[Anonymous], 2017, R LANG ENV STAT COMP
[3]   Plant Microbiome Engineering: Expected Benefits for Improved Crop Growth and Resilience [J].
Arif, Inessa ;
Batool, Maria ;
Schenk, Peer M. .
TRENDS IN BIOTECHNOLOGY, 2020, 38 (12) :1385-1396
[4]   Effects of multiple N, P, and K fertilizer combinations on strawberry growth and the microbial community [J].
Bai, Xue ;
Liu, Kaili ;
Ning, Tiao ;
Deng, Changjun ;
Wang, Li ;
Li, Diyan ;
Wang, Tao ;
Li, Jing .
PLOS ONE, 2023, 18 (11)
[5]   Ecological principles underlying the increase of productivity achieved by cereal-grain legume intercrops in organic farming. A review [J].
Bedoussac, Laurent ;
Journet, Etienne-Pascal ;
Hauggaard-Nielsen, Henrik ;
Naudin, Christophe ;
Corre-Hellou, Guenaelle ;
Jensen, Erik Steen ;
Prieur, Loic ;
Justes, Eric .
AGRONOMY FOR SUSTAINABLE DEVELOPMENT, 2015, 35 (03) :911-935
[6]   Intercropping promotes the ability of durum wheat and chickpea to increase rhizosphere phosphorus availability in a low P soil [J].
Betencourt, Elodie ;
Duputel, Marek ;
Colomb, Bruno ;
Desclaux, Dominique ;
Hinsinger, Philippe .
SOIL BIOLOGY & BIOCHEMISTRY, 2012, 46 :181-190
[7]  
Callahan BJ, 2016, NAT METHODS, V13, P581, DOI [10.1038/NMETH.3869, 10.1038/nmeth.3869]
[8]   Responses of soil bacterial community and Cd phytoextraction to a Sedum alfredii-oilseed rape (Brassica napus L. and Brassica juncea L.) intercropping system [J].
Cao, Xuerui ;
Luo, Jipeng ;
Wang, Xiaozi ;
Chen, Zhiqin ;
Liu, Guoqun ;
Khan, Muhammad Bilal ;
Kang, Kyong Ju ;
Feng, Ying ;
He, Zhenli ;
Yang, Xiaoe .
SCIENCE OF THE TOTAL ENVIRONMENT, 2020, 723
[9]   Modulation of Maize Rhizosphere Microbiota Composition by Inoculation with Azospirillum argentinense Az39 (Formerly A. brasilense Az39) [J].
Coniglio, Anahi ;
Larama, Giovanni ;
Molina, Romina ;
Mora, Veronica ;
Torres, Daniela ;
Marin, Anelis ;
Ignacio Avila, Andres ;
Lede NoirCarlan, Coline ;
Erijman, Leonardo ;
Lucia Figuerola, Eva ;
de Souza, Emanuel Maltempi ;
Cassan, Fabricio .
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION, 2022, 22 (03) :3553-3567
[10]   The Dynamics of Phosphorus Uptake and Remobilization during the Grain Development Period in Durum Wheat Plants [J].
El Mazlouzi, Mohamed ;
Morel, Christian ;
Robert, Thierry ;
Chesseron, Coralie ;
Salon, Christophe ;
Cornu, Jean-Yves ;
Mollier, Alain .
PLANTS-BASEL, 2022, 11 (08)