Genome-wide Identification and Expression Analysis of the Argonaute Gene Family in Sugar Beet (Beta vulgaris L.)

被引：0

作者：

Fu, Zengjuan ^{[1
,2
]}

Wang, Liang ^{[1
,2
]}

Li, Xiaodong ^{[1
,2
]}

E, Yuanyuan ^{[1
,2
]}

Liang, Yahui ^{[1
,2
]}

Zhang, Bizhou ^{[1
,2
]}

Zhao, Shangmin ^{[1
,2
]}

Zhang, Hui ^{[1
,2
]}

Zheng, Wenzhe ^{[1
,2
]}

Bai, Chen ^{[1
,2
]}

Sun, Mengyuan ^{[1
,2
]}

Zhang, Ziqiang ^{[1
,2
]}

Chang, Yue ^{[1
,2
]}

机构：

[1] Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010031, Peoples R China

[2] Inner Mongolia Acad Agr & Anim Husb Sci, Inner Mongolia Key Lab Sugar Beet Genet & Germplas, Hohhot 010031, Peoples R China

来源：

SUGAR TECH | 2025年

关键词：

Sugar beet; Argonaute protein; Gene family; Gene expression; STRUCTURAL BASIS; RNA; RECOGNITION;

D O I：

10.1007/s12355-025-01597-z

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The argonaute (AGO) protein is a component of the transcription-induced silencing complex of target genes mediated by the small RNA pathway in plants, which, together with small RNAs, regulates gene expression and influences, among other processes, the formation of monoembryos and polyembryos in plants. In this work, a comprehensive systematic identification of the BvAGO gene family in the whole genome of sugar beet was carried out by utilizing the conserved domains of the AGO proteins. A total of nine BvAGO gene family members were identified, and the physicochemical properties, phylogeny, gene structure, promoter regions, and replication events of the nine family members were analyzed via bioinformatics methods. Moreover, the expression profiles of the nine genes of the AGO family in beet were analyzed in different beet tissues and verified via RT-qPCR technology. The results revealed that these BvAGO proteins were classified into four branches with protein lengths ranging from 712 to 1142 aa, and subcellular localization predicted that four BvAGO members were located in the cytoplasm, four in the nucleus, and one in the extracellular region and that the nine genes were distributed on five chromosomes. Overall, the expression patterns of sugar beet AGO gene members and Arabidopsis thaliana AGO gene members in tissues were very similar, among which the BvAGO1, BvAGO4c, BvAGO5a, and BvAGO5b genes were highly expressed in all the tissues; these genes are hypothesized to be involved in the regulation of related single-embryo multiple-embryo development in sugar beets, and the present study provides guidance for further studies of the function of AGO proteins in sugar beets. This study provides guidance for further research on the function of the AGO protein in sugar beet.

引用

页数：12

共 27 条

[1] GTSF1 accelerates target RNA cleavage by PIWI-clade Argonaute proteins [J].

Arif, Amena ;

Bailey, Shannon ;

Izumi, Natsuko ;

Anzelon, Todd A. ;

Ozata, Deniz M. ;

Andersson, Cecilia ;

Gainetdinov, Ildar ;

MacRae, Ian J. ;

Tomari, Yukihide ;

Zamore, Phillip D. .

NATURE, 2022, 608 (7923) :618-+

[2] Role of RNA Interference (RNAi) in the Moss Physcomitrella patens [J].

Arif, Muhammad Asif ;

Frank, Wolfgang ;

Khraiwesh, Basel .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2013, 14 (01) :1516-1540

[3] Genome-wide identification of Dicer-like, Argonaute and RNA-dependent RNA polymerase gene families and their expression analyses in response to viral infection and abiotic stresses in Solanum lycopersicum [J].

Bai, Miao ;

Yang, Guo-Shun ;

Chen, Wen-Ting ;

Mao, Zhen-Chuan ;

Kang, Hou-Xiang ;

Chen, Guo-Hua ;

Yang, Yu-Hong ;

Xie, Bing-Yan .

GENE, 2012, 501 (01) :52-62

[4] Two ARGONAUTE proteins loaded with transposon-derived small RNAs are associated with the reproductive cell lineage in Arabidopsis [J].

Bradamante, Gabriele ;

Nguyen, Vu Hoang ;

Incarbone, Marco ;

Meir, Zohar ;

Bente, Heinrich ;

Dona, Mattia ;

Lettner, Nicole ;

Scheid, Ortrun Mittelsten ;

Gutzat, Ruben .

PLANT CELL, 2024, :863-880

[5] TBtools-II: A "one for all, all for one"bioinformatics platform for biological big-data mining [J].

Chen, Chengjie ;

Wu, Ya ;

Li, Jiawei ;

Wang, Xiao ;

Zeng, Zaohai ;

Xu, Jing ;

Liu, Yuanlong ;

Feng, Junting ;

Chen, Hao ;

He, Yehua ;

Xia, Rui .

MOLECULAR PLANT, 2023, 16 (11) :1733-1742

[6] Gene Silencing of Argonaute5 Negatively Affects the Establishment of the Legume-Rhizobia Symbiosis [J].

del Rocio Reyero-Saavedra, Maria ;

Qiao, Zhenzhen ;

del Socorro Sanchez-Correa, Maria ;

Enrique Diaz-Pineda, M. ;

Reyes, Jose L. ;

Covarrubias, Alejandra A. ;

Libault, Marc ;

Valdes-Lopez, Oswaldo .

GENES, 2017, 8 (12)

[7] Structural basis for 5′-nucleotide base-specific recognition of guide RNA by human AGO2 [J].

Frank, Filipp ;

Sonenberg, Nahum ;

Nagar, Bhushan .

NATURE, 2010, 465 (7299) :818-822

[8] A Multigenic Network of ARGONAUTE4 Clade Members Controls Early Megaspore Formation in Arabidopsis [J].

Hernandez-Lagana, Elvira ;

Rodriguez-Leal, Daniel ;

Lua, Judith ;

Vielle-Calzada, Jean-Philippe .

GENETICS, 2016, 204 (03) :1045-+

[9] Genome-wide identification, organization and phylogenetic analysis of Dicer-like, Argonaute and RNA-dependent RNA Polymerase gene families and their expression analysis during reproductive development and stress in rice [J].

Kapoor, Meenu ;

Arora, Rita ;

Lama, Tenisha ;

Nijhawan, Aashima ;

Khurana, Jitendra P. ;

Tyagi, Akhilesh K. ;

Kapoor, Sanjay .

BMC GENOMICS, 2008, 9 (1)

[10] The role of ARGONAUTE1 (AGO1) in meristem formation and identity [J].

Kidner, CA ;

Martienssen, RA .

DEVELOPMENTAL BIOLOGY, 2005, 280 (02) :504-517

← 1 2 3 →