Electrochemical Aptasensor Based on Topological Material Bi2Se3 Sheets for Sensitive Detection of Interferon-γ

被引：0

作者：

Li, Xiuxia ^{[1
,2
]}

Bai, Jiangyue ^{[1
,2
]}

Liang, Lin ^{[3
]}

Jiang, Yujiu ^{[1
,2
]}

Zhu, Peng ^{[1
,2
]}

Wang, Zhiwei ^{[1
,2
]}

Xu, Shiqi ^{[1
,2
]}

Zhang, Chunpan ^{[1
,2
]}

Yao, Qing'er ^{[4
]}

Hu, Deng ^{[1
,2
]}

Li, Shanshan ^{[3
]}

Han, Junfeng ^{[1
,2
]}

机构：

[1] Beijing Inst Technol, Ctr Quantum Phys, Sch Phys, Key Lab Adv Optoelect Quantum Architecture & Measu, Beijing 100081, Peoples R China

[2] Beijing Inst Technol, Int Ctr Quantum Mat, Zhuhai 519000, Peoples R China

[3] China Japan Friendship Hosp, Dept Rheumatol, Beijing 100029, Peoples R China

[4] Beijing Inst Technol, Yangtze Delta Reg Acad, Jiaxing 314019, Peoples R China

来源：

ACS APPLIED BIO MATERIALS | 2025年 / 8卷 / 04期

基金：

中国国家自然科学基金; 北京市自然科学基金;

关键词：

IFN-gamma; topological materials; Bi2Se3; sheets; aptasensor; electrochemicaldetection; GOLD; SURFACE; DISULFIDES; ELECTRODE;

D O I：

10.1021/acsabm.5c00081

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Interferon-gamma (IFN-gamma), an essential inflammatory cytokine, is intricately associated with a variety of fatal diseases as a key early biomarker. In this work, we designed and constructed an electrochemical aptasensor based on topological insulator Bi2Se3 sheets. Micron-scale Bi2Se3 sheets were prepared by electrochemical exfoliation from single crystals to make electrodes of the aptasensors. The unique and robust Dirac surface states of Bi2Se3 could enhance the charge transfer efficiency of the solid-liquid interface, improving the performance of the aptasensors. The developed aptasensor exhibits a linear response to IFN-gamma concentration in the range of 1-100 pg/mL with a detection limit as low as 0.6 pg/mL, enabling it to meet the clinical requirements. The performance of the aptasensors also shows excellent stability and selectivity. Furthermore, the aptasensor was applied to human serum detection and was comparable in performance to the clinical standard enzyme-linked immunosorbent assay technique. Our work indicates that the aptasensor based on Bi2Se3 sheets has great potential for application in the clinical detection of IFN-gamma and other possible biomarkers.

引用

页码：3300 / 3308

页数：9

共 47 条

[1] A triple-helix molecular switch-based electrochemical aptasensor for interferon-gamma using a gold electrode and Methylene Blue as a redox probe [J].