Hawthorn pectin mitigates high-fat diet induced hyperlipidemia in mice through promoting Dubosiella newyorkensis

被引：0

作者：

Zhao, Qianyun ^{[1
,2
]}

Liu, Yubin ^{[1
]}

Li, Mingxing ^{[1
,3
]}

Zhao, Long ^{[4
]}

Wang, Tiangang ^{[4
]}

Xiao, Yaqin ^{[5
]}

Wei, Shulin ^{[1
]}

Wu, Ke ^{[1
]}

Yang, Jiayue ^{[1
]}

Wang, Yaqiu ^{[6
]}

Zhao, Yueshui ^{[1
,3
]}

Du, Fukuan ^{[1
,3
]}

Chen, Yu ^{[1
,3
]}

Deng, Shuai ^{[1
,3
]}

Shen, Jing ^{[1
,3
]}

Xiao, Zhangang ^{[1
,3
,9
]}

Li, Zhi ^{[4
,7
]}

Wang, Shengpeng ^{[5
]}

Wu, Xu ^{[1
,8
,10
]}

机构：

[1] Southwest Med Univ, Sch Pharm, Dept Pharmacol, Cell Therapy & Cell Drugs Luzhou Key Lab, Luzhou 646000, Sichuan, Peoples R China

[2] Maternal & Child Hlth Care & Family Planning Serv, Dept Pharm, Meishan 620000, Sichuan, Peoples R China

[3] South Sichuan Inst Translat Med, Luzhou 646000, Sichuan, Peoples R China

[4] Southwest Med Univ, Affiliated Tradit Chinese Med Hosp, Dept Spleen & Stomach Dis, Luzhou 646000, Sichuan, Peoples R China

[5] Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau 999078, Peoples R China

[6] Northeast Forestry Univ, Aulin Coll, Haerbin 150040, Peoples R China

[7] Southwest Med Univ, Affiliated Tradit Chinese Med Hosp, Key Lab Integrated Tradit Chinese & Western Med P, Luzhou 646000, Sichuan, Peoples R China

[8] Luzhou Peoples Hosp, Dept Paediat, Luzhou 646000, Sichuan, Peoples R China

[9] Sichuan Coll Tradit Chinese Med, Sch Pharm, Mianyang 621000, Sichuan, Peoples R China

[10] Luzhou Peoples Hosp, Dept Paediat Care, Luzhou 646000, Sichuan, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2025年 / 359卷

关键词：

Hawthorn pectin; Hyperlipidemia; Dubosiella newyorkensis; Lactobacillus; RG-I; Structure-activity relationship; GUT MICROBIOTA; EXTRACTION;

D O I：

10.1016/j.carbpol.2025.123570

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Hawthorn fruit is rich in pectins; however, the gut microbiota-modulating effects of hawthorn pectin remain unclear. In this study, enzyme-extracted hawthorn pectin (Mw: 126.24 kDa; HG/RG-I ratio: 55.64 %/37.93 %; branching degree: 2.44) was fractionated into two distinct components: D1 (spherical, 345.76 kDa, HG/RG-I 57.48 %/37.25 %, branching degree 2.16) and D2 (flexible, 17.20 kDa, HG/RG-I 78.24 %/21.76 %, branching degree 1.33). Hawthorn pectin directly stimulated Dubosiella newyorkensis (D. newyorkensis) and Bifidobacterium bifidum (B. bifidum) growth in vitro. In hyperlipidemic mice, it alleviated dyslipidemia and enriched the abundance of Lactobacillus and Dubosiella. Parallel experiments confirmed that D. newyorkensis supplementation similarly alleviated dyslipidemia and increased Lactobacillus abundance, which was mediated by bacterial acetate production. Notably, only D1 emulated the pectin's prebiotic activity, promoting D. newyorkensis and B. bifidum growth. The high-RG-I D1, with elevated branching and larger Mw, was identified as the key driver of hawthorn pectin's prebiotic effect. These findings highlight that hawthorn pectin, particularly D1, specifically targets probiotic D. newyorkensis to synergize with Lactobacillus against hyperlipidemia.

引用

页数：18

共 43 条

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