AUNIP was a candidate marker for prognosis and immunology in pan-cancer

被引：0

作者：

Xiaorong Guo ^{[1
]}

Ting Liu ^{[2
]}

Nan Li ^{[3
]}

Li Jin ^{[4
]}

机构：

[1] The Second Affiliated Hospital of Harbin Medical University,Department of Pathology

[2] Beijing Ditan Hospital,Department of Pathology

[3] Capital Medical University,Department of Pathology

[4] The Fourth Affiliated Hospital of Harbin Medical University,Cancer Center, Department of Pathology

[5] Zhejiang Provincial People’s Hospital,undefined

[6] Affiliated People’s Hospital,undefined

[7] Hangzhou Medical College,undefined

来源：

3 Biotech | 2025年 / 15卷 / 6期

关键词：

AUNIP; Pan-cancer; Prognostic; Immunity; Hepatocellular carcinoma;

D O I：

10.1007/s13205-025-04294-6

中图分类号：

学科分类号：

摘要：

AUNIP (Aurora kinase A[Aurora-A] and ninein-interacting protein), is a key factor regulating the end-state of DNA cleavage. It has been reported that AUNIP affects the progression of some tumors; however, the molecular functions involved in AUNIP remain unknown. We employed some databases, such as TCGA, GTEx, TIMER, GEPIA2, cBioportal, and GSCALite, to study AUNIP gene expression, prognosis, gene variation, and drug sensitivity. The relationship between AUNIP and clinicopathological information was explored using Wilcoxon test. The association between AUNIP and TMB, MSI, immunocyte infiltration, and immune checkpoints were analyzed using Spearman correlation analysis. We employed GSEA to research the functional mechanisms involved in AUNIP for pan-cancer. Moreover, we conducted immunohistochemistry (IHC) to investigate AUNIP difference expression between liver hepatocellular carcinoma (LIHC) and normal tissues. The Chisq test was used to study the correlation of AUNIP with clinical characteristics. AUNIP was highly expressed in majority of tumors and IHC analysis demonstrated that AUNIP expression was higher in LIHC than normal tissues. AUNIP overexpression had adverse outcomes in adrenocortical carcinoma (ACC), brain lower grade glioma (LGG), LIHC, mesothelioma (MESO), and sarcoma (SARC). Furthermore, high AUNIP expression led to unfavorable prognosis in LIHC. AUNIP was associated with T stage, N stage, and clinicopathological analysis in several cancers and AUNIP expression had a correlation with histologic grade in LIHC by IHC. Mutation analysis showed that AUNIP was the highest frequency of genetic changes in cholangiocarcinoma (CHOL). AUNIP was negatively associated with 30 small-molecule drugs that inhibit tumor development. AUNIP expression had association with TMB, MSI, immune cell infiltration, and immune checkpoints for various tumors. GSEA results suggested that AUNIP mainly participated in the cell cycle, DNA replication, mismatch repair, and homologous recombination.Pan-cancer study considered AUNIP as a potential prognostic marker and high latent diagnostic biomarker.

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