Flavin-Containing Monooxygenases Mediate Resistance to Nereistoxin Insecticides in Lepidopteran Pests: Insights into Conserved Tertiary Amine Oxidation Mechanisms

被引：0

作者：

Rao, Cong ^{[1
]}

Yuan, Chao ^{[1
]}

He, Wangjin ^{[1
]}

Guo, Hailiang ^{[1
]}

Liu, Kuitun ^{[1
]}

Fan, Jianfeng ^{[1
]}

Su, Jianya ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Plant Protect, State Key Lab Agr & Forestry Biosecur, Nanjing 210095, Jiangsu, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2025年 / 73卷 / 13期

基金：

中国国家自然科学基金;

关键词：

flavin-containing monooxygenase; nereistoxin; cartap; monosultap; insecticide metabolism; insecticide resistance; CHILO-SUPPRESSALIS LEPIDOPTERA; DEPENDENT MONOOXYGENASE; EVOLUTIONARY RECRUITMENT; METAFLUMIZONE RESISTANCE; PYRROLIZIDINE ALKALOIDS; BEET ARMYWORM; STEM BORER; POPULATIONS; METABOLISM; PLANT;

D O I：

10.1021/acs.jafc.5c01818

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

This study elucidates the molecular mechanism by which flavin-containing monooxygenase (FMO) mediates metabolic resistance to nereistoxin insecticides in lepidopteran pests. A field population of Spodoptera exigua exhibited 50-fold resistance with upregulated SeFMO expression. Using FMO-specific inhibitors, recombinant protein expression, and mass spectrometry, we confirmed that FMO catalyzes N-oxidation of nereistoxin insecticide at the tertiary amine nitrogen. Molecular docking revealed that insect FMO's catalytic mechanism resembles that of human FMO. Transgenic Drosophila models demonstrated that the FMO-mediated N-oxidation enhances insecticide resistance, indicating evolutionary conservation. This highlights FMO's role in insecticide detoxification and its conserved function across species, providing new insights into pest resistance mechanisms.

引用

页码：7704 / 7715

页数：12

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