Development and application of sex-specific indel markers for Hippophae salicifolia based on third-generation sequencing

被引：0
作者：
Zeng, Zhefei [1 ,2 ]
Zhang, Shutong [1 ]
Tan, Xin [1 ]
Tso, Norzin [1 ]
Shang, Zhuo [1 ]
Zhang, Jifeng [1 ]
Li, Wei [1 ,2 ]
Wang, Junwei [1 ,2 ]
Zhang, Wenju [3 ,4 ]
Qiong, La [1 ,2 ]
机构：
[1] Tibet Univ, Sch Ecol & Environm, Key Lab Biodivers & Environm Qinghai Tibetan Plate, Minist Educ, Lhasa, Peoples R China
[2] Tibet Univ, Yani Wetland Ecosyst Positioning Observat & Res St, Nyingchi, Peoples R China
[3] Fudan Univ, Sch Life Sci, Inst Biodivers Sci, State Key Lab Genet Eng,Minist Educ,Key Lab Biodiv, Shanghai, Peoples R China
[4] Fudan Univ, Inst Plant Biol, Sch Life Sci, Shanghai, Peoples R China
来源：
BMC PLANT BIOLOGY | 2025年 / 25卷 / 01期
关键词：
Hippophae salicifolia; Sex determination; Molecular markers; Sex chromosomes; IDENTIFICATION; VALIDATION;
D O I：
10.1186/s12870-025-06725-1
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Hippophae salicifolia, a dioecious shrub endemic to the Himalayan region, holds substantial ecological and economic value. The lack of pre-flowering morphological traits for sex identification has long impeded efficient germplasm management and breeding efforts. In this study, we utilized third-generation sequencing technology to conduct whole-genome comparative analysis of known-sex individuals, identifying insertion/deletion loci significantly associated with sex. Based on these loci, we designed a specific primer pair, Hsa09. PCR amplification results demonstrated 100% accuracy in sex differentiation, with female individuals showing a single band and male individuals exhibiting a double-band pattern. Cross-species validation revealed limited applicability of Hsa09 in closely related Hippophae species, suggesting notable divergence in sex determination mechanisms within the genus. This study establishes the first molecular tool for early sex identification in H. salicifolia, overcoming the dependence on reproductive organ development in traditional methods. It provides essential technical support for understanding the evolutionary pathways of sex chromosomes and advancing sex-regulated breeding strategies within the Hippophae genus.
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页数：8
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