Advances in DNA damage detection: Current progress, challenges, and future directions

被引：0

作者：

Kang, Heeseok ^{[1
,2
]}

Park, Hyung Joon ^{[1
,2
]}

Kang, Jieun ^{[3
]}

Hwang, Yuna ^{[3
,4
]}

Lee, Yongjin ^{[1
,2
]}

Park, Sang-Uk ^{[5
]}

Kim, Jeongkyu ^{[5
]}

Lee, Kwan Hyi ^{[1
,2
]}

Jang, Sunbok ^{[3
,4
]}

机构：

[1] Korea Inst Sci & Technol KIST, Ctr Adv Biomol Recognit, Biomed Res Div, Seoul 02792, South Korea

[2] Korea Univ, KU KIST Grad Sch Converging Sci & Technol, Seoul 02841, South Korea

[3] Ewha Womans Univ, Grad Sch Pharmaceut Sci, Coll Pharm, Seoul 03760, South Korea

[4] Ewha Womans Univ, Grad Program Innovat Biomat Convergence, Seoul 03760, South Korea

[5] Chung Ang Univ, Dept Life Sci, Seoul 06794, South Korea

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2025年 / 189卷

基金：

新加坡国家研究基金会;

关键词：

DNA damage; DNA repair; Biomarkers; Measurement techniques; Personalized medicine; Biosensors; CYCLOBUTANE PYRIMIDINE DIMERS; BASE-EXCISION-REPAIR; MOLECULAR MARKER; ABASIC SITES; IN-VIVO; FLUORESCENCE DETECTION; GLYCOSYLASE ACTIVITY; SENSITIVE DETECTION; MASS-SPECTROMETRY; OXIDATIVE DAMAGE;

D O I：

10.1016/j.trac.2025.118246

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

DNA is much continuously exposed to endogenous and exogenous agents that induce damage, potentially leading to genomic instability, disease development, and aging. Detecting DNA damage and understanding associated repair mechanisms are essential for advancing diagnostic techniques, biosensors, and therapeutic interventions. Since DNA damage varies, the desired measurement approach differs, highlighting the need for an unbiased exploration of DNA damage biomarkers, along with a critical analysis of various quantification methods. Therefore, this review aims to provide a comprehensive overview of current methodologies for measuring DNA damage, focusing on direct detection of DNA lesions and indirect measurement of repair enzymes. We discuss various analytical, biochemical, and imaging techniques, identify the limitations in existing technologies and suggest future directions. This review emphasizes the growing need for advanced tools to measure DNA damage, which is set to play a transformative role in early disease detection, optimizing treatments, and supporting safe human space exploration.

引用

页数：18

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