Na-Ag Ion-Exchanged Glass Substrates for Plasmon-Enhanced Fluorescence Imaging of Neutrophils

被引：0

作者：

Inozemtsev, Vladimir A. ^{[1
]}

Dokukin, Maxim E. ^{[2
]}

Sgibnev, Yevgeniy M. ^{[2
]}

Sherstyukova, Ekaterina A. ^{[1
]}

Kandrashina, Snezhanna S. ^{[1
]}

Shvedov, Mikhail A. ^{[1
]}

Shelaev, Artem V. ^{[2
]}

Nikonorov, Nikolay V. ^{[3
]}

Sergunova, Viktoria A. ^{[1
]}

Baryshev, Alexander V. ^{[2
]}

机构：

[1] VA Negovsky Res Inst Gen Reanimatol, Fed Res & Clin Ctr Intens Care Med & Rehabilitol, 25 Petrovka St Bldg 2, Moscow 107031, Russia

[2] Dukhov Automat Res Inst VNIIA, 22 Sushchevskaya St, Moscow 127055, Russia

[3] ITMO Univ, Res Ctr Opt Mat, 49 Kronverksky Ave, St Petersburg 197101, Russia

来源：

SENSORS | 2025年 / 25卷 / 07期

关键词：

ion exchange; plasmonic nanostructures; silver nanoparticles; neutrophil; fluorescence enhancement and lifetime; 2-DIMENSIONAL ARRAYS; GOLD NANOPARTICLES; SILVER; RESONANCE; ABSORPTION;

D O I：

10.3390/s25072278

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Here, we study the fluorescence response of neutrophils stained with the wheat germ agglutinin Alexa Fluor 594 dye when the cells are placed on plasmonic nanoparticle substrates. Specifically, we focused on gold and silver nanoparticles with particle sizes ranging from 12 to 250 nm. It was demonstrated that the intensity of fluorescence can be increased by more than 10 times when using substrates with silver nanoparticles formed by Na+-Ag+ ion exchange in glass. The fluorescence enhancement depends significantly on both the size and surface density of the silver nanoparticles and the membrane staining procedure.

引用

页数：15

共 61 条

[1] Surface plasmon resonance in gold nanoparticles: a review [J].