Unraveling the immunogenic landscape: A quest for B-cell linear epitopes in the African swine fever virus H339R protein

被引：0

作者：

Chen, Zhuting ^{[1
]}

Xin, Cheng ^{[2
]}

Zhang, Shuang ^{[1
]}

Zhan, Qinyi ^{[1
]}

Zhang, Fei ^{[1
]}

Liu, Yanli ^{[1
]}

Yang, Xin ^{[1
]}

Yang, Xiaojun ^{[1
]}

Ren, Zhouzheng ^{[1
]}

机构：

[1] College of Animal Science and Technology, Northwest A&F University, Shaanxi, Yangling

[2] School of Life Sciences, Zhengzhou University, Henan, Zhengzhou

来源：

International Journal of Biological Macromolecules | 2025年 / 310卷

关键词：

African swine fever virus; B-cell epitopes; Diagnostic tools; H339R protein; Monoclonal antibodies; Vaccines;

D O I：

10.1016/j.ijbiomac.2025.142944

中图分类号：

学科分类号：

摘要：

African swine fever (ASF) is a highly contagious viral disease causing severe economic losses in the global swine industry. The development of effective diagnostic tools and vaccines is crucial for the control and prevention of ASF. In this study, we aimed to identify and characterize linear B-cell epitopes within the African swine fever virus (ASFV) H339R protein, a potential diagnostic and vaccine target. Using monoclonal antibodies (mAbs) and enzyme-linked immunosorbent assay (ELISA)-based mapping, we identified four distinct linear B-cell epitopes in the H339R protein. These epitopes exhibited specific reactivity with ASFV-positive swine sera in western blot (WB) and indirect ELISA assays. Alanine-scanning mutagenesis revealed critical amino acid residues within each epitope that contribute to antibody recognition. The identification and characterization of these linear B-cell epitopes in the ASFV H339R protein provide valuable insights into the antigenic structure of the virus and lay the foundation for the development of novel diagnostic tools and epitope-based vaccines for ASF. These findings contribute to control and prevent this economically significant disease and to safeguard the global swine industry. © 2025

引用

共 37 条

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