An experiment was conducted to examine spectrally based factors that contribute to the visual perception of interior environments, with a focus on brightness perception. Thirty-two participants evaluated 60 different lighting scenes in a mock office. The lighting spectral power distributions varied systematically in illuminance, chromaticity (CCT and Duv), s cone opic daylight efficacy ratio (sc-DER), melanopic daylight efficacy ratio (mel-DER), and color rendition (Rf, Rg, and Rcs,h1). At the operationalized levels of these variables, illuminance had the largest effect on brightness perception. Notably, the second largest effect was due to changes in red chroma (Rcs,h1). The effect of sc-DER was also statistically significant but was a tertiary effect. The effects of mel-DER, CCT, and Duv were not statistically significant. This large effect of red chroma is consistent with the existing understanding that changes in color perception are often perceived when illuminance changes. With appropriate changes in color rendition and other factors held constant, spatial brightness perception was preserved through a decrease from 500 lux to 250 lux.