Investigation of Oxidative-Stress Impact on Human Osteoblasts During Orthodontic Tooth Movement Using an In Vitro Tension Model

被引:0
|
作者
Hosseini, Samira [1 ]
Diegelmann, Julia [2 ]
Folwaczny, Matthias [2 ]
Sabbagh, Hisham [1 ]
Otto, Sven [3 ]
Kakoschke, Tamara Katharina [3 ]
Wichelhaus, Andrea [1 ]
Baumert, Uwe [1 ]
Rankovic, Mila Janjic [1 ]
机构
[1] Ludwig Maximilians Univ Munchen, LMU Univ Hosp, Dept Orthodont & Dentofacial Orthoped, D-80336 Munich, Germany
[2] Ludwig Maximilians Univ Munchen, LMU Univ Hosp, Dept Conservat Dent & Periodontol, D-80336 Munich, Germany
[3] Ludwig Maximilians Univ Munchen, LMU Univ Hosp, Dept Oral & Maxillofacial Surg & Facial Plast Surg, D-80337 Munich, Germany
关键词
human osteoblasts; tensile strain; oxidative stress; bone remodeling; orthodontic tooth movement; PERIODONTAL-LIGAMENT CELLS; APOPTOSIS; GENES; PCR; INTERLEUKIN-8; INFLAMMATION; MECHANISMS; CROSSTALK; AUTOPHAGY; CYTOKINES;
D O I
10.3390/ijms252413525
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In recent years, there has been a growing number of adult orthodontic patients with periodontal disease. The progression of periodontal disease is well-linked to oxidative stress (OS). Nevertheless, the impact of OS on orthodontic tooth movement (OTM) is not fully clarified. Therefore, we applied an OS in vitro-model utilizing H2O2 to study its effect on tension-induced mechanotransduction in human osteoblasts (hOBs). Experimental parameters were established based on cell viability and proliferation. Apoptosis detection was based on caspase-3/7 activity. Gene expression related to bone-remodeling (RUNX2, P2RX7, TNFRSF11B/OPG), inflammation (CXCL8/IL8, IL6, PTRGS2/COX2), autophagy (MAP1LC3A/LC3, BECN1), and apoptosis (CASP3, CASP8) was analyzed by RT-qPCR. IL6 and PGE2 secretion were determined by ELISA. Tension increased the expression of PTRGS2/COX2 in all groups, especially after stimulation with higher H2O2 concentration. This corresponds also to the measured PGE2 concentrations. CXCL8/IL8 was upregulated in all groups. Cells subjected to tension alone showed a general upregulation of osteogenic differentiation-related genes; however, pre-stimulation with OS did not induce significant changes especially towards downregulation. MAP1LC3A/LC3, BECN1 and CASP8 were generally upregulated in cells without OS pre-stimulation. Our results suggest that OS might have considerable impacts on cellular behavior during OTM.
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页数:22
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