Identification of single nucleotide polymorphisms and candidate genes associated with fiber content in sweetpotato (Ipomoea batatas (L.) Lam.) through a genome-wide association study

被引：0
作者：
Tae Hwa Kim [1 ]
Kyo Hwui Lee [1 ]
Mi Nam Chung [1 ]
Hyeong-Un Lee [1 ]
Sang-Sik Nam [1 ]
Won Park [1 ]
机构：
[1] Value Crop Research Institute, National Institute of Crop and Food Science, RDA, Muan
来源：
BMC Plant Biology | / 25卷 / 1期
关键词：
Candidate genes; Fiber content; GWAS; SNP; Sweetpotato;
D O I：
10.1186/s12870-025-06614-7
中图分类号：
学科分类号：
摘要：
Background: Sweetpotato (Ipomoea batatas (L.) Lam.) is an essential root crop with several nutritional benefits, including high dietary fiber content. While fiber contributes positively to human health by reducing the risk of metabolic and gastrointestinal diseases, excessive fiber accumulation can negatively impact texture and consumer preference. Despite its importance, the genetic mechanisms underlying fiber content in sweetpotato remain largely unexplored. Therefore, this study aimed to identify the genomic regions and candidate genes associated with fiber content through a genome-wide association study (GWAS). Results: Significant phenotypic variation in fiber content were observed among 140 sweetpotato genotypes. The GWAS analysis identified seven significant single nucleotide polymorphisms (SNPs), with Iba_chr07a_20294133 and Iba_chr12a_38616338 consistently detected across the FarmCPU and BLINK models. Notably, three SNPs (Iba_chr01a_17621178, Iba_chr10a_773882, and Iba_chr12a_38616338) showed significant phenotypic differentiation between homozygous alleles, making them promising candidates for marker development. Candidate gene analysis identified four genes with significantly upregulated expression in high-fiber genotypes: IbANT1 (adenine nucleotide transporter BT1), IbCYP86B1 (cytochrome P450 86B1), IbSCR3 (scarecrow-like protein 3), and IbFER (FERONIA receptor-like kinase). These genes are involved in suberin biosynthesis, cell wall remodeling, and metabolic regulation, suggesting their crucial roles in fiber accumulation. Conclusion: This study provides novel insights into the genetic regulation of fiber content in sweetpotato. The identification of significant SNPs and candidate genes offers valuable resources for breeding programs targeting fiber optimization. Further validation is essential for the effective application of these SNPs and genes into marker-assisted selection strategies. © The Author(s) 2025.
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  • [1] Alam M.K., A comprehensive review of sweet potato (Ipomoea batatas [L]. Lam): Revisiting the associated health benefits, Trends Food Sci Technol, 115, pp. 512-529, (2021)
  • [2] Dincer C., Karaoglan M., Erden F., Tetik N., Topuz A., Ozdemir F., Effects of baking and boiling on the nutritional and antioxidant properties of sweet potato [Ipomoea batatas (L.) Lam.] cultivars, Plant Foods Hum Nutr, 66, pp. 341-347, (2011)
  • [3] Xu X., Wu S., Chen K., Zhang H., Zhou S., Lv Z., Et al., Comprehensive evaluation of raw eating quality in 81 sweet potato (Ipomoea batatas (L.) Lam) varieties, Foods, 12, (2023)
  • [4] Allan M.C., Johanningsmeier S.D., Nakitto M., Guambe O., Abugu M., Pecota K.V., Et al., Baked sweetpotato textures and sweetness: An investigation into relationships between physicochemical and cooked attributes, Food Chem X, 21, (2024)
  • [5] Turner N.D., Lupton J.R., Dietary fiber, Adv Nutr, 2, pp. 151-152, (2011)
  • [6] Cheng L., Zhang X., Hong Y., Li Z., Li C., Gu Z., Characterisation of physicochemical and functional properties of soluble dietary fibre from potato pulp obtained by enzyme-assisted extraction, Int J Biol Macromol, 101, pp. 1004-1011, (2017)
  • [7] Liu M., Zhou S., Li Y., Tian J., Zhang C., Structure, physicochemical properties and effects on nutrients digestion of modified soluble dietary fiber extracted from sweet potato residue, Food Res Int, 150, (2021)
  • [8] Zou X., Dai K., Zhang M., Zhang R., Jia X., Dong L., Et al., Dietary fiber from sweet potato residue with different processing methods: Physicochemical, functional properties, and bioactivity in vitro, LWT, 206, (2024)
  • [9] Sun Q., Zhang W., Huang Q., Zeng Y., Zeng X., Fan J., Evaluation of physicochemical properties and in vitro starch digestion of noodles enriched with thermally assisted potassium carbonate treated sweet potato residue, LWT, 182, (2023)
  • [10] Nakitto M., Johanningsmeier S.D., Moyo M., Bugaud C., de Kock H., Dahdouh L., Et al., Sensory guided selection criteria for breeding consumer-preferred sweetpotatoes in Uganda, Food Qual Preference, 101, (2022)
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