Exosomes from miR-21-5p-modified adipose-derived stem cells promote wound healing by regulating M2 macrophage polarization in a rodent model of pressure ulcer

被引：0

作者：

Su, Yongsheng ^{[1
]}

Huang, Zhibin ^{[1
]}

Chen, Yuanwen ^{[1
]}

Deng, Jingcheng ^{[1
]}

Huang, Yubin ^{[1
]}

Xiong, Wei ^{[2
]}

机构：

[1] Peoples Hosp Baoan Shenzhen, Dept Burn & Plast Surg, Shenzhen 518000, Peoples R China

[2] Huazhong Univ Sci & Technol, Union Shenzhen Hosp, Dept Burn & Plast Surg, 89 Taoyuan Rd, Shenzhen 518000, Guangdong, Peoples R China

来源：

JOURNAL OF MOLECULAR HISTOLOGY | 2025年 / 56卷 / 03期

关键词：

Pressure ulcers; ADSCs; Exosomes; miR-21-5p; Macrophage; Polarization; GENE-EXPRESSION; REPAIR;

D O I：

10.1007/s10735-025-10407-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Pressure ulcers represent a significant healthcare burden worldwide. Numerous research has demonstrated the therapeutic potential of adipose-derived stem cell (ADSC)-derived exosomes in promoting wound healing. This study aims to investigate whether exosomes derived from miRNA-modified ADSCs play a role in pressure ulcers by affecting inflammation and macrophage polarization. ADSCs were identified by detecting the surface markers and multilineage differentiation potential. Lentiviruses carrying miR-21-5p were transduced in ADSCs for stable overexpression. Exosomes were extracted from ADSCs and identified. RT-qPCR was employed to detect RNA levels. A mouse model of pressure ulcers was established, followed by injection of exosomes. DiO staining was conducted to assess exosome biodistribution at wound sites. Hematoxylin-eosin and Masson staining were conducted for histological analysis. Immunofluorescence staining was used to evaluate TNF-alpha and IL-6 expression in mouse wound tissues. Western blotting was conducted to evaluate protein levels of macrophage polarization markers in vivo and in vitro. The results revealed that exosomes derived from miR-21-5p-overexpressing ADSCs promoted wound healing and reduced inflammatory cytokine expression in mouse wound tissues. Moreover, exosomal miR-21-5p induced macrophage M2 polarization in both mouse wound tissues and bone marrow-derived macrophages. Mechanistically, exosomal miR-21-5p inhibited NF-kappa B signal transduction in mouse wound tissues. In conclusion, ADSC-derived exosomes promote M2 macrophage polarization and inhibit inflammatory response in pressure ulcers via miR-21-5p delivery.

引用

页数：12