The Co-Incorporation of Zn/Cu or Zn/Co Ions Improves the Bone Regeneration Potential of PEOT/PBT-βTCP Composite 3D-Printed Scaffolds

被引：0
作者：
Nikody, Martyna [1 ,2 ]
Li, Jiaping [1 ,2 ]
Koper, David [1 ,2 ,3 ]
Balmayor, Elizabeth R. [4 ]
Habibovic, Pamela [1 ]
Moroni, Lorenzo [2 ]
机构：
[1] Maastricht Univ, MERLN Inst Technol Inspired Regenerat Med, Dept Instructive Biomat Engn, NL-6211 Maastricht, Netherlands
[2] Maastricht Univ, MERLN Inst Technol Inspired Regenerat Med, Dept Complex Tissue Regenerat, Universiteitssingel 40, NL-6229 ER Maastricht, Netherlands
[3] Maastricht Univ, Dept Cranio Maxillofacial Surg, Med Ctr, P Debyelaan 25, NL-6229 HX Maastricht, Netherlands
[4] RWTH Aachen Univ Hosp, Dept Orthopaed, Expt Orthopaed & Trauma Surg, Trauma, Pauwelsstr 30, D-52074 Aachen, Germany
来源：
ADVANCED NANOBIOMED RESEARCH | 2025年 / 5卷 / 03期
关键词：
bones; composite scaffolds; scaffolds; tissue regeneration; trace elements; MESENCHYMAL STEM-CELLS; OSTEOGENIC DIFFERENTIATION; BIOACTIVE MOLECULES; GROWTH-FACTORS; ZINC; COATINGS; ANGIOGENESIS; DEFECTS; BIOMATERIALS; MECHANISMS;
D O I：
10.1002/anbr.202400139
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Treatment of critical-sized bone defects remains challenging despite bone's regenerative capacity. Herein, a combination of a biodegradable polymer possessing bone-bonding properties with bioactive beta-tricalcium phosphate (beta TCP) particles coated with osteogenic (Zinc) and angiogenic (copper or cobalt) ions has been proposed. beta TCP was coated with zinc and copper (Zn/Cu) or zinc and cobalt (Zn/Co) using 15 mM (low) or 45 mM (high) metallic ion solutions. Composites were obtained by a combination of the beta TCP with poly(ethylene oxide terephthalate)/poly(butylene terephthalate) (PEOT/PBT) copolymer in a 50:50 ratio. Composites were additively manufactured into 3D porous scaffolds and their osteogenic and angiogenic properties evaluated using a direct culture with human mesenchymal stromal cells (hMSCs) as well as an indirect coculture with human umbilical vein endothelial cells (HUVECs). We hypothesized that the combination of Zn/Cu or Zn/Co in the form of a coating of the beta TCP particles would stimulate both osteogenic and angiogenic properties of PEOT/PBT-beta TCP scaffolds. In addition, we investigated whether the resulting biomaterials influenced the paracrine function of hMSCs. Zn/Cu or Zn/Co were successfully co-incorporated into the ceramic without changing its chemistry. Scaffolds containing low concentrations of Zn/Co increased the expression of RUNX2, OCN, and OPN, while scaffolds with low concentrations of Zn/Cu enhanced the expression of ALPL. On the protein level, high Zn/Co concentrations elevated ALP and collagen production. Angiogenic properties improved with increased VEGFA expression by hMSCs and branching of tubules formed by HUVECs, particularly with low concentrations of Zn/Co. Scaffolds with high ion concentrations also increased cytokine and chemokine secretion, suggesting enhanced paracrine effects.
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页数：17
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