Chrysanthemum zawadskii var. latilobum Flower Essential Oil Reduces MRSA Pathogenicity by Inhibiting Virulence Gene Expression

被引：1
作者：
Kim, Ji-Hee [1 ,2 ]
Park, Bog-Im [3 ]
Kim, Young-Hoi [4 ]
Yoon, Ji-Su [2 ]
Choi, Na-Young [5 ]
Kim, Kang-Ju [6 ]
机构：
[1] Wonkwang Univ, Sch Food, Dept Convergence Technol Food Ind, Iksan 54538, Jeonbuk, South Korea
[2] Korea Univ, Grad Sch, Dept Hlth & Safety Convergence Sci, Transdisciplinary Major Learning Hlth Syst, Seoul 02841, South Korea
[3] Kunsan Natl Univ, Sch Food, Dept Food & Nutr, Kunsan 54150, Jeonbuk, South Korea
[4] Jeonbuk Natl Univ, Coll Agr & Life Sci, Dept Food Sci & Technol, Jeonju 54896, Jeonbuk, South Korea
[5] Wonkwang Univ, Coll Educ, Iksan 54538, Jeonbuk, South Korea
[6] Wonkwang Univ, Sch Dent, Dept Oral Microbiol, Iksan 54538, Jeonbuk, South Korea
来源：
MOLECULES | 2025年 / 30卷 / 03期
关键词：
<italic>Chrysanthemum zawadskii</italic>; MRSA; antimicrobial; biofilm; virulence; RESISTANT STAPHYLOCOCCUS-AUREUS; ANTIBIOTIC-RESISTANCE; INFECTIONS; CLINDAMYCIN; VANCOMYCIN; CYTOKINE; IMPACT;
D O I：
10.3390/molecules30030553
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The essential oil extracted from the flowers of Chrysanthemum zawadskii var. latilobum (Maxim.) Kitam (CZEO), family Asteraceae, was investigated to determine its ability to inhibit the pathogenicity of methicillin-resistant Staphylococcus aureus (MRSA). The chemical composition of CZEO was analyzed using gas chromatography-flame ionization detector and gas chromatography-mass spectrometry, and 88 compounds were identified and categorized as monoterpenes (68.82%), sesquiterpenes (17.82%), and others (5.01%). CZEO inhibited MRSA floating cell growth, acid production, and biofilm formation in a concentration-dependent manner. Furthermore, confocal laser scanning and scanning electron microscopy confirmed that the CZEO treatment decreased MRSA viability and notably reduced the three-dimensional density of the biofilm. Real-time PCR demonstrated that the mRNA expression of the MRSA gene A (mecA), accessory gene regulator A (agrA), staphylococcal accessory regulator A (sarA), and staphylococcal enterotoxin A (sea), which are pivotal genes implicated in MRSA pathogenicity, declined in a concentration-dependent manner following the CZEO treatment compared with the control. Thus, CZEO appeared to directly target the pathogenicity MRSA regulators. These findings substantiate the potential of CZEO as a natural antimicrobial agent for preventing MRSA infections.
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页数：23
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