Preparation and Characterization of Demineralized Bone Extracellular Matrix/Chitosan Porous Microcarriers

被引：0
作者：
Jiang, Jie [1 ]
Fang, Jianjun [1 ]
Liu, Wenjuan [2 ]
Guo, Meimei [1 ]
Lu, Zhongjia [1 ]
Tang, Heng [1 ]
Lan, Yi [3 ]
Zhou, Zhihua [1 ,2 ,4 ]
机构：
[1] Hunan Univ Sci & Technol, Sch Chem & Chem Engn, Xiangtan, Peoples R China
[2] Hunan Univ Sci & Technol, Hunan Prov Key Lab Controllable Preparat & Funct A, Xiangtan, Peoples R China
[3] Guangdong Gaoshun Environm Technol Co Ltd, Hunan Branch, Xiangtan, Peoples R China
[4] Hunan Univ Sci & Technol, Hunan Prov Coll Key Lab Mol Design & Green Chem, Xiangtan, Peoples R China
来源：
JOURNAL OF MACROMOLECULAR SCIENCE PART B-PHYSICS | 2024年
关键词：
Cell cytoxicity; chitosan; decellularized bone extracellular matrix; porous microcarriers; IN-VITRO; TISSUE; MICROSPHERES; SCAFFOLDS; MATRIX;
D O I：
10.1080/00222348.2024.2409526
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Composite porous microcarriers based on demineralized bone extracellular matrix (DBECM) and chitosan (Cs) were prepared using a combined emulsion and freeze-drying method. The effects of digestion by pepsin on the DBECM properties were investigated. The morphologies and porous structure of the DBECM/Cs porous microcarriers were observed by using a scanning electron microscope (SEM). The cytocompatibility of the DBECM/Cs porous microcarriers were studied by using cell counting kit-8 (CCK-8) and calcein acetoxymethyl ester/propidium iodide (CAM/PI). DBECM was digested into small pieces with a loose porous sponge structure and the digested DBECM showed an amorphous state. The resultant DBECM/Cs porous microcarriers showed a spherical shape and an interconnective porous structure. The CCK-8 and CAM/PI analyses indicated that the DBECM/Cs porous microcarriers were non-cytotoxic and showed good biocompatibility, providing a more favorable growth environment for cells.
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页数：13
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