Extracellular Vesicles From Bone Marrow-Derived Macrophages Enriched in ARG1 Enhance Microglial Phagocytosis and Haematoma Clearance Following Intracerebral Haemorrhage

被引：0

作者：

Hu, Libin ^{[1
,2
,3
]}

Chen, Zihang ^{[1
,2
,3
]}

Lu, Jianglong ^{[4
]}

Jiang, Shandong ^{[1
,2
]}

Lin, Haopu ^{[1
,2
]}

Zhou, Jiayin ^{[1
,2
]}

Wang, Ning ^{[4
]}

Ding, Chao ^{[4
]}

Ni, Weifang ^{[4
]}

Peng, Haitao ^{[4
]}

Li, Yin ^{[1
,2
]}

He, Xuchao ^{[1
,2
]}

Li, Jianru ^{[1
,2
]}

Jing, Chaohui ^{[5
]}

Cao, Yang ^{[6
]}

Zhou, Hang ^{[1
,2
,3
]}

Yan, Feng ^{[1
,2
,3
]}

Chen, Gao ^{[1
,2
,3
]}

机构：

[1] Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Neurosurg, Hangzhou, Peoples R China

[2] Zhejiang Key Lab Res & Transformat Major Neurosurg, Hangzhou, Peoples R China

[3] State Key Lab Transvasc Implantat Devices, Hangzhou, Peoples R China

[4] Zhejiang Univ, Sch Med, Hangzhou, Zhejiang, Peoples R China

[5] Shanghai Jiao Tong Univ, XinHua Hosp, Dept Neurosurg, Sch Med, Shanghai, Peoples R China

[6] Westlake Univ, Affiliated Hangzhou Peoples Hosp 1, Sch Med, Dept Neurosurg, Hangzhou, Peoples R China

来源：

JOURNAL OF EXTRACELLULAR VESICLES | 2025年 / 14卷 / 01期

基金：

中国国家自然科学基金;

关键词：

ARG1; BMDM; EVs; ICH; microglia; phagocytosis; EXOSOMES; RECEPTOR; ERYTHROPHAGOCYTOSIS; EFFEROCYTOSIS; ACTIVATION; RESOLUTION; DISEASE; TOOLS; CELLS; GAMMA;

D O I：

10.1002/jev2.70041

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Microglial phagocytosis of haematomas is crucial for neural functional recovery following intracerebral haemorrhage (ICH), a process regulated by various factors from within and outside the central nervous system (CNS). Extracellular vesicles (EVs), significant mediators of intercellular communication, have been demonstrated to play a pivotal role in the pathogenesis and progression of CNS diseases. However, the regulatory role of endogenous EVs on the phagocytic capacity of microglia post-ICH remains elusive. Utilising multi-omics analysis of brain tissue-derived EVs proteomics and single-cell RNA sequencing, this study identified that bone marrow-derived macrophages (BMDMs) potentially enhance microglial phagocytosis via EVs following ICH. By blocking BMDMs and reducing ARG1 in BMDM-derived EVs, we demonstrated that BMDMs facilitate erythrophagocytosis by delivering ARG1 to microglia via EVs post-ICH. EVs-carried ARG1 was found to augment phagocytosis by promoting RAC1-dependent cytoskeletal remodelling in microglia. Collectively, this research uncovers an intercellular communication pathway from BMDMs to microglia mediated by EVs post-ICH. This provides a novel paradigm for EV-mediated intercellular communication mechanisms and suggests a promising therapeutic potential for BMDM-derived EVs in the treatment of ICH.

引用

页数：24

共 3 条

[1] Extracellular vesicles derived from M1 macrophages enhance rat midpalatal suture expansion by promoting initial bone turnover and inflammation
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PROGRESS IN ORTHODONTICS, 2023, 24 (01)
[2] Exosomes Derived from MicroRNA-146a-5p-Enriched Bone Marrow Mesenchymal Stem Cells Alleviate Intracerebral Hemorrhage by Inhibiting Neuronal Apoptosis and Microglial M1 Polarization
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[3] MicroRNA-421-3p-abundant small extracellular vesicles derived from M2 bone marrow-derived macrophages attenuate apoptosis and promote motor function recovery via inhibition of mTOR in spinal cord injury
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JOURNAL OF NANOBIOTECHNOLOGY, 2020, 18 (01)

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