Involvement of AhR/ARNT in tolerance to deltamethrin by regulating the expression of TcGSTs6 in Tribolium castaneum

被引：0

作者：

Luo, Zhichao ^{[1
]}

Chen, Haoting ^{[2
]}

Guan, Daojie ^{[1
]}

Hang, Chen ^{[1
]}

Wang, Zhichao ^{[1
]}

Ge, Huichen ^{[1
]}

Qian, Kun ^{[1
]}

Wang, Jianjun ^{[1
]}

机构：

[1] Yangzhou Univ, Coll Plant Protect, Yangzhou 225009, Peoples R China

[2] Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Peoples R China

来源：

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | 2025年 / 208卷

关键词：

Tribolium castaneum; AhR; ARNT; GSTs; Deltamethrin; Transcriptional regulation; ARYL-HYDROCARBON RECEPTOR; GLUTATHIONE-S-TRANSFERASES; MULTIPLE SEQUENCE ALIGNMENT; RESISTANCE; DIOXIN; ACTIVATION; AHR; MECHANISM;

D O I：

10.1016/j.pestbp.2024.106281

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is well known that the transcription factors aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) play important roles in the detoxification of xenobiotic compounds including insecticides. In this study, TcAhR and TcARNT were cloned from Tribolium castaneum. RT-qPCR analysis revealed that TcAhR and TcARNT were highly expressed in brain and epidermis of female adults and upregulated after exposure of the adults to deltamethrin. Knockdown of TcAhR and TcARNT increased the susceptibility of T. castaneum to deltamethrin and decreased the enzymatic activity of Glutathione S-transferase (GST). Consistently, knockdown of TcAhR and TcARNT led to the downregulation of TcGSTs6 and suppressed the induction of TcGSTs6 by deltamethrin treatment. Increased susceptibility of T. castaneum to deltamethrin was also observed after knockdown of TcGSTs6. Further dual-luciferase reporter assay revealed that co-overexpression of TcAhR and TcARNT in Sf9 cells significantly enhanced the promoter activity of TcGSTs6. These results provided insights into the function of insect AhR and ARNT as well as the regulatory mechanisms of insect GSTs.

引用

页数：9

共 52 条

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