Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique

被引：0

作者：

Yamamoto, Tomofumi ^{[1
,2
,3
]}

Urabe, Fumihiko ^{[2
,4
]}

Yoshioka, Yusuke ^{[1
]}

Yamamoto, Yusuke ^{[2
]}

Ochiya, Takahiro ^{[1
]}

机构：

[1] Tokyo Med Univ, Inst Med Sci, Dept Mol & Cellular Med, Tokyo, Japan

[2] Natl Canc Ctr, Lab Integrat Oncol, Tokyo, Japan

[3] Natl Inst Hlth Sci, Div Biol Chem & Biol, Kawasaki, Kanagawa, Japan

[4] Jikei Univ, Sch Med, Dept Urol, Tokyo, Japan

来源：

STAR PROTOCOLS | 2025年 / 6卷 / 01期

关键词：

Cell Biology; Cell-based Assays; Molecular Biology;

D O I：

10.1016/j.xpro.2024.103569

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Extracellular vesicles (EVs) play a key role in cancer development and cellular homeostasis by transferring the biological cargo to recipient cells. Here, we describe steps for screening EV secretion-related genes by combining a micro- RNA (miRNA) library and ExoScreen, a highly sensitive EV detection technique. We also detail procedures for screening the direct target genes regulated by miRNAs. This protocol provides a useful tool for understanding complex intracellular communications involved in EV secretion. For complete details on the use and execution of this protocol, please refer to Yamamoto et al.1

引用

页数：17

共 3 条

[1] Oncogene-regulated release of extracellular vesicles
Kilinc, Seda
Paisner, Rebekka
Camarda, Roman
Gupta, Suprit
Momcilovic, Olga
Kohnz, Rebecca A.
Avsaroglu, Baris
L'Etoile, Noelle D.
Perera, Rushika M.
Nomura, Daniel K.
Goga, Andrei
[J]. DEVELOPMENTAL CELL, 2021, 56 (13) : 1989 - +
[2] The miR-1908/SRM regulatory axis contributes to extracellular vesicle secretion in prostate cancer
Urabe, Fumihiko
Kosaka, Nobuyoshi
Sawa, Yurika
Ito, Kagenori
Kimura, Takahiro
Egawa, Shin
Ochiya, Takahiro
Yamamoto, Yusuke
[J]. CANCER SCIENCE, 2020, 111 (09) : 3258 - 3267
[3] Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progression
Yamamoto, Tomofumi
Nakayama, Jun
Urabe, Fumihiko
Ito, Kagenori
Nishida-Aoki, Nao
Kitagawa, Masami
Yokoi, Akira
Kuroda, Masahiko
Hattori, Yutaka
Yamamoto, Yusuke
Ochiya, Takahiro
[J]. CELL REPORTS, 2024, 43 (08):

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