Identification of key genes and signaling pathways in coconut (Cocos nucifera L.) under drought stress via comparative transcriptome analysis

BackgroundDrought stress has become a pervasive environmental challenge, significantly impacting all stages of plant growth and development under changing climatic conditions worldwide. In coconut, drought stress critically impairs reproductive development, notably reducing the quality of pollen and gametes during fertilization. Therefore, the seedlings of the aromatic coconut variety were subjected to drought stress for varying durations: control (no stress), 7 days, 14 days, and 21 days to find the potential molecular mechanisms and genes related to coconut drought tolerance through transcriptomic analysis. Our study may provide a theoretical basis for investigations into drought stress tolerance that will be useful for further coconut improvement.ResultsWe assessed antioxidant enzyme activity and conducted comparative transcriptome analyses of aromatic coconut under different drought conditions (7, 14, and 21 days). Our findings revealed significant rises in superoxide dismutase (SOD), peroxidase (POD) activities and proline (Pro) content across all drought periods compared to control plants, suggesting that these enzymes play a crucial role in the adaptive response of coconuts to drought stress. RNA-seq data identified 280, 729, and 6,698 differentially expressed genes (DEGs) at 7, 14, and 21 days, respectively. Principal Component Analysis (PCA) revealed that coconut samples were scattered and separated across different treatment points, suggesting the presence of differentially expressed genes (DEGs), particularly in the 21 day drought treatment (GH21d). KEGG pathway analysis indicated that DEGs were significantly enriched in pathways related to plant-pathogen interaction, plant hormone signaling, and mitogen-activated protein kinase (MAPK) signaling. Functional annotation of these DEGs revealed key candidate genes involved in several hormone signaling pathways, including abscisic acid (ABA), jasmonates (JA), auxin (AUX), brassinosteroids (BR), ethylene (ET), and gibberellin (GA), along with MAPK pathway which may regulate plant adaptation to drought stress through processes such as plant growth, cell division, stomatal closure, root growth, and stomatal development. This study provides valuable insights into the genetic and molecular basis of drought tolerance in coconuts, paving the way for the improvement of drought-tolerant coconut varieties.ConclusionsUnder drought stress, the expression of genes related to plant growth, stomatal closure, cell division, stress response, adaptation, and stomatal development appears to play a critical role in drought tolerance in coconut. Our results revealed that multiple genes may contribute to the drought tolerance mechanism in coconut through various hormone signaling pathways, including ABA, JA, auxin, BR, GA, and ethylene. These findings offer new insights into the key molecular mechanisms governing drought tolerance in aromatic coconut. Furthermore, the candidate genes and pathways identified in this study could be valuable for developing strategies to enhance drought tolerance in coconut plants.Clinical trial numberNot Applicable.