Magnetisation transfer, T1 and T2*relaxation in canine menisci of elderly dogs-an ex vivo study in stifle joints

被引：0
作者：
Bunzendahl, Lena [1 ,2 ]
Moussavi, Amir [2 ,3 ]
Bleyer, Martina [4 ]
Neumann, Stephan [1 ]
Boretius, Susann [2 ]
机构：
[1] Georg August Univ Gottingen, Inst Vet Med, Small Anim Clin, Gottingen, Germany
[2] German Primate Ctr, Leibniz Inst Primate Res, Funct Imaging Lab, Gottingen, Germany
[3] Univ Appl Sci, Fac hsch Sudwestfalen, Hagen, Germany
[4] German Primate Ctr, Leibniz Inst Primate Res, Pathol Unit, Gottingen, Germany
来源：
FRONTIERS IN VETERINARY SCIENCE | 2025年 / 12卷
关键词：
multi-parameter MRI; T2*mapping; T1; mapping; magnetisation transfer; canine menisci; osteoarthritis; stifle joint; proteoglycans; ARTICULAR-CARTILAGE; KNEE CARTILAGE; RELAXATION-TIMES; T2; MRI; OSTEOARTHRITIS; INJURY; DEGENERATION; LESIONS;
D O I：
10.3389/fvets.2025.1521684
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Magnetic resonance imaging (MRI) is widely used in human medicine, offering multiple contrast mechanisms to visualise different tissue types. It is also gaining importance in veterinary medicine, including diagnosing joint disorders. The menisci of the stifle joint play a crucial role in the development of osteoarthritis (OA), and multi-parameter MRI of the menisci may aid in early OA diagnosis, potentially improving therapeutic outcomes. In a previous ex vivo study, we measured T2 relaxation times in menisci of elderly dogs with mild histological signs of degeneration but no clinical symptoms of lameness. As no significant changes in T2 relaxation times were observed in relation to histological scores, the present study extends this investigation by exploring more advanced MR parameters-including T1 relaxation time, T2* relaxation time, magnetisation transfer ratio (MTR), and magnetisation transfer saturation (MTsat)-to assess their potential for detecting early microstructural changes in the menisci. While T2* relaxation times and MTR showed no significant variation across histological scores, MTsat values increased with higher proteoglycan staining. In contrast, the apparent T1 relaxation time (T1app) was lower in menisci with elevated proteoglycan scores and increased with higher cellularity scores. The correlation between MTsat and proteoglycan content suggests that MTsat, along with T1app, could be a promising parameter for characterising the extracellular matrix. However, further research is needed to validate these findings.
引用
收藏
页数：10
相关论文
共 53 条
  • [1] Pownder S.L., Hayashi K., Caserto B.G., Breighner R.E., Norman M.L., Potter H.G., Et al., Quantitative magnetic resonance imaging and histological comparison of Normal canine menisci, Vet Comp Orthop Traumatol, 31, pp. 452-457, (2018)
  • [2] McDermott I., Meniscal tears, repairs and replacement: their relevance to osteoarthritis of the knee, Br J Sports Med, 45, pp. 292-297, (2011)
  • [3] Hu J., Xin H., Chen Z., Zhang Q., Peng Y., Jin Z., The role of menisci in knee contact mechanics and secondary kinematics during human walking, Clin Biomech (Bristol, Avon), 61, pp. 58-63, (2019)
  • [4] Fox A.J.S., Wanivenhaus F., Burge A.J., Warren R.F., Rodeo S.A., The human meniscus: a review of anatomy, function, injury, and advances in treatment, Clin Anat, 28, pp. 269-287, (2015)
  • [5] Zarins Z.A., Bolbos R.I., Pialat J.B., Link T.M., Li X., Souza R.B., Et al., Cartilage and meniscus assessment using T1rho and T2 measurements in healthy subjects and patients with osteoarthritis, Osteoarthr Cartil, 18, pp. 1408-1416, (2010)
  • [6] Lee W., Miller E.Y., Zhu H., Luetkemeyer C.M., Schneider S.E., Neu C.P., High frame rate deformation analysis of knee cartilage by spiral dualMRI and relaxation mapping, Magn Reson Med, 89, pp. 694-709, (2023)
  • [7] Welsch G.H., Apprich S., Zbyn S., Mamisch T.C., Mlynarik V., Scheffler K., Et al., Biochemical (T2, T2* and magnetisation transfer ratio) MRI of knee cartilage: feasibility at ultra-high field (7T) compared with high field (3T) strength, Eur Radiol, 21, pp. 1136-1143, (2011)
  • [8] Mittal S., Pradhan G., Singh S., Batra R., T1 and T2 mapping of articular cartilage and menisci in early osteoarthritis of the knee using 3-tesla magnetic resonance imaging, Pol J Radiol, 84, pp. 549-e564, (2019)
  • [9] Eijgenraam S.M., Bovendeert F.A.T., Verschueren J., van Tiel J., Bastiaansen-Jenniskens Y.M., Wesdorp M.A., Et al., T2 mapping of the meniscus is a biomarker for early osteoarthritis, Eur Radiol, 29, pp. 5664-5672, (2019)
  • [10] Hofmann F.C., Neumann J., Heilmeier U., Joseph G.B., Nevitt M.C., McCulloch C.E., Et al., Conservatively treated knee injury is associated with knee cartilage matrix degeneration measured with MRI-based T2 relaxation times: data from the osteoarthritis initiative, Skeletal Radiol, 47, pp. 93-106, (2018)
123456