A G-quadruplex-based method to detect ClO− in the rostral ventrolateral medulla of stress-induced hypertension by self-complementary DNA probe

被引：0

作者：

Haisheng Liu ^{[1
]}

Hongyu Wang ^{[1
]}

Chao Zhang ^{[1
]}

Meng Wang ^{[1
]}

Qian Liu ^{[1
]}

Yaru Zhao ^{[1
]}

Jiaxing Yu ^{[1
]}

Guihong Zhao ^{[1
]}

Ming Zhang ^{[1
]}

机构：

[1] College of Agriculture and Bioengineering, Heze University, Shandong, Heze

来源：

Microchimica Acta | 2025年 / 192卷 / 5期

基金：

中国国家自然科学基金;

关键词：

Fluorescence spectroscopy; G-quadruplex/ThT; Hypochlorite; Phosphorothioate oligonucleotides; Rostral ventrolateral medulla; Stress-induced hypertension;

D O I：

10.1007/s00604-025-07080-6

中图分类号：

学科分类号：

摘要：

A G-quadruplex-based fluorescent biosensor is introduced that employs a self-complementary DNA probe for detection of hypochlorite ions (ClO−) in the rostral ventrolateral medulla (RVLM) of rats afflicted with stress-induced hypertension (SIH). The biosensor capitalizes on the interaction between Thioflavin T (ThT) and guanine-rich DNA sequences, exhibiting exceptional sensitivity, specificity, and swift detection capabilities for ClO−. Through optimization of the detection parameters, we achieved a minimal detection threshold of 0.0486 μM and a rapid reaction time of 5 min. The biosensor’s high selectivity for ClO− was confirmed by its lack of response to other reactive oxygen species (ROS). When applied to SIH rat models, the biosensor revealed markedly elevated ClO− levels in the RVLM of SIH rats versus control rats, implicating ClO− in the disease’s pathophysiology. This label-free, G-quadruplex-based biosensor represents a significant advancement in the detection of ClO⁻, offering a highly sensitive and specific tool for monitoring reactive oxygen species in biological systems. This study not only deepens our comprehension of SIH but also offers a potent diagnostic and monitoring tool for ROS-related diseases, with implications for therapeutic intervention and disease management. © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2025.

引用

共 29 条

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