Whole genome sequencing and genome characterization of Aichivirus isolated from Korean adults

被引：0
作者：
Woo, Seoyoung [1 ]
Hossain, Md Iqbal [1 ]
Jung, Soontag [1 ]
Yeo, Daseul [1 ]
Yoon, Danbi [1 ]
Hwang, Seongwon [1 ]
Do, Hee-Jung [2 ]
Eyun, Seong-il [2 ]
Choi, Changsun [1 ]
机构：
[1] Chung Ang Univ, Coll Biotechnol & Nat Resources, Dept Food & Nutr, Anseong, South Korea
[2] Chung Ang Univ, Coll Nat Sci, Dept Life Sci, Seoul, South Korea
来源：
JOURNAL OF MEDICAL VIROLOGY | 2024年 / 96卷 / 09期
基金：
新加坡国家研究基金会;
关键词：
aichivirus; nanopore sequencing; phylogenetic analysis; sanger sequencing; whole genome sequencing; VIRUS; 1; PHYLOGENETIC ANALYSIS; WEB SERVER; GENERATION; GASTROENTERITIS; RECOMBINATION; SUBSTITUTION; ALGORITHM; INFERENCE; SELECTION;
D O I：
10.1002/jmv.29902
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The whole-genome sequence (WGS) analysis of Aichivirus (AiV) identified in Korea was performed in this study. Using Sanger and Nanopore sequencing, the 8228-nucleotide-long genomic sequence of AiV (OQ121963) was determined and confirmed to belong to genotype A. The full-length genome of OQ121963 consisted of a 7296 nt open reading frame (ORF) that encodes a single polyprotein, and 5 ' UTR (676 nt) and 3 ' UTR (256 nt) at 5 ' and 3 ' ends, respectively. The ORF consisted of leader protein (L), structural protein P1 (VP0, VP1, and VP3), and nonstructural protein P2 (2A, 2B, and 2C) and P3 (3A, 3B, 3C, and 3D). The secondary structure analysis of the 5 ' UTR identified only stem-loop C (SL-C) and not SL-A and SL-B. The variable region of the AiV genome was analyzed by MegAlign Pro and reconfirmed by SimPlot analysis using 16 AiV whole genomes known to date. Among the entire regions, structural protein region P1 showed the lowest amino acid identity (96.07%) with reference sequence AB040749 (originated in Japan; genotype A), while the highest amino acid identity (98.26%) was confirmed in the 3D region among nonstructural protein region P2 and P3. Moreover, phylogenetic analysis of the WGS of OQ121963 showed the highest homology (96.96%) with JX564249 (originated in Taiwan; genotype A) and lowest homology (90.14%) with DQ028632 (originated in Brazil; genotype B). Therefore, the complete genome characterization of OQ121963 and phylogenetic analysis of the AiV conducted in this study provide useful information allowing to improve diagnostic tools and epidemiological studies of AiVs.
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页数：12
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