Functional characterization of peroxiredoxin 5 from yellowtail clownfish (Amphiprion clarkii): Immunological expression assessment, antioxidant activities, heavy metal detoxification, and nitrosative stress mitigation

被引：1

作者：

Rodrigo, D. C. G. ^{[1
,2
]}

Udayantha, H. M. V. ^{[1
,2
]}

Liyanage, D. S. ^{[1
,2
]}

Omeka, W. K. M. ^{[1
,2
]}

Kodagoda, Y. K. ^{[1
,2
]}

Hanchapola, H. A. C. R. ^{[1
,2
]}

Dilshan, M. A. H. ^{[1
,2
]}

Ganepola, G. A. N. P. ^{[1
,2
]}

Warnakula, W. A. D. L. R. ^{[1
,2
]}

Kim, Gaeun ^{[1
,2
]}

Kim, Jeongeun ^{[1
,2
]}

Lee, Jihun ^{[1
,2
]}

Wan, Qiang ^{[1
,2
,3
]}

Lee, Jehee ^{[1
,2
,3
]}

机构：

[1] Jeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea

[2] Jeju Natl Univ, Ctr Genom Select Korean Aquaculture, Jeju 63243, South Korea

[3] Jeju Natl Univ, Marine Life Res Inst, Jeju 63333, South Korea

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2025年 / 162卷

基金：

新加坡国家研究基金会;

关键词：

Amphiprion clarkii; Peroxiredoxin; 5; Antioxidant; Oxidative stress; Innate immune response; ATYPICAL 2-CYS PEROXIREDOXIN; INDUCED OXIDATIVE STRESS; ENZYME; OVEREXPRESSION; GENE; IDENTIFICATION; INVOLVEMENT; APOPTOSIS; DEFENSE; CLONING;

D O I：

10.1016/j.dci.2024.105289

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Peroxiredoxin 5 (Prdx5) is the last recognized member of Prdx family. It is a unique, atypical, 2-Cys antioxidant enzyme, protecting cells from death caused by reactive oxygen species (ROS). In this study, the Prdx5 ortholog of Amphiprion clarkii (AcPrdx5) was identified and characterized to explore its specific structural features and functional properties. The open reading frame of AcPrdx5 is 573 bp long and encodes 190 amino acids containing a mitochondrial targeting sequence, thioredoxin domain, and two conserved cysteine residues responsible for antioxidant function. The predicted molecular weight and theoretical isoelectric point of AcPrdx5 are 20.3 kDa and 9.01, respectively. AcPrdx5 sequences were found to be highly conserved across the other orthologs from various organisms and it distinctively clustered within the fish Prdx5 subclade of the phylogenetic tree. The expression of AcPrdx5 was ubiquitously detected among twelve tested tissues, with the highest level in the brain. Furthermore, the mRNA levels of AcPrdx5 in the blood and head-kidney tissues were significantly (p < 0.05) upregulated following polyinosinic-polycytidylic acid (Poly I:C), lipopolysaccharide (LPS), and Vibrio harveyi immune challenge. A concentration-dependent antioxidant potential of recombinant AcPrdx5 was observed in insulin disulfide bond reduction, heavy metal detoxification, free radical and hydrogen peroxide (H2O2) scavenging assays. Additionally, AcPrdx5 overexpression in fathead minnow (FHM) cells upregulated the antioxidant-associated gene (Rrm1, MAPK, SOD2, and PRDX1) expression after H2O2 treatment, and promoted cell viability upon arsenic (As) exposure. In macrophages, AcPrdx5 overexpression effectively suppressed substantial nitric oxide production under lipopolysaccharide treatment. Collectively, our results suggest that AcPrdx5 may play roles in both antioxidant defense system and innate immune response against pathogenic invasions in A. clarkii.

引用

页数：13

共 65 条

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