C2C12 myoblasts differentiate into myofibroblasts via the TGF-β1 signaling pathway mediated by Fibulin2

被引：2

作者：

Yang, Yongqiang ^{[1
]}

Li, Lei ^{[2
]}

Fei, Jun ^{[3
]}

Li, Zhong ^{[1
]}

机构：

[1] Southwest Med Univ, Dept Orthopaed, Affiliated Hosp, Luzhou, Sichuan, Peoples R China

[2] Army Med Univ, Daping Hosp, Dept Stem Cell & Regenerat Med, State Key Lab Trauma Burn & Combined Injury, Chongqing, Peoples R China

[3] Army Med Univ, Daping Hosp, Dept Emergency, State Key Lab Trauma Burn & Combined Injury, Chongqing 400042, Peoples R China

来源：

GENE | 2025年 / 936卷

关键词：

Myofibroblasts; Fibulin2; TGF-beta; 1; STELLATE CELL ACTIVATION; SKELETAL-MUSCLE; SATELLITE CELLS; EXPRESSION;

D O I：

10.1016/j.gene.2024.149048

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Myoblasts play a critical role in the regeneration of skeletal muscle following injury. It has been reported that local elevation of transforming growth factor-(31 (TGF-(31) after skeletal muscle injury induces differentiation of myoblasts into myofibroblasts. However, the mechanisms underlying this differentiation process remain incompletely understood. In this study, we found that Fibulin2 expression significantly increases in myoblasts in response to TGF-(31 stimulation. Elevated Fibulin2 levels enhance the expression of fibrotic markers. Conversely, downregulation of Fibulin2 in myoblasts inhibits the upregulation of fibrotic markers induced by TGF-(31 stimulation. Extracellular secretion of Fibulin2 activates the TGF-(31-Smad2 pathway, thereby promoting the upregulation of fibrotic markers. Hence, Fibulin2 and TGF-(31 form a positive feedback loop that facilitates differentiation of myoblasts into myofibroblasts.

引用

页数：16

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