Purification of Self-Assembled DNA Tetrahedra Using Gel Electrophoresis

被引：1
作者：
Patel, Akul [1 ]
Valsangkar, Vibhav [1 ]
Halvorsen, Ken [1 ]
Chandrasekaran, Arun Richard [1 ]
机构：
[1] SUNY Albany, RNA Inst, Albany, NY 12222 USA
来源：
CURRENT PROTOCOLS | 2022年 / 2卷 / 09期
关键词：
DNA nanotechnology; DNA tetrahedra; gel electrophoresis; purification; self-assembly; NANOSTRUCTURES;
D O I：
10.1002/cpz1.560
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA nanostructures have found applications in a variety of fields such as biosensing, drug delivery, cellular imaging, and computation. Several of these applications require purification of the DNA nanostructures once they are assembled. Gel electrophoresis-based purification of DNA nanostructures is one of the methods used for this purpose. Here, we describe a step-by-step protocol for a gel-based method to purify self-assembled DNA tetrahedra. With further optimization, this method could also be adapted for other DNA nanostructures. (c) 2022 Wiley Periodicals LLC.
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页数：10
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