Transcriptomic Approaches to Investigate the Anti-Aging Effects of Blueberry Anthocyanins in a Caenorhabditis Elegans Aging Model

被引:1
作者
Ding, Jie [1 ]
Liu, Jiahui [1 ]
Guo, Qingqi [1 ]
Zhang, Na [2 ]
机构
[1] Northeast Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China
[2] Harbin Univ Commerce, Coll Food Engn, Harbin 150028, Peoples R China
基金
中国国家自然科学基金;
关键词
blueberry anthocyanins; caenorhabditis elegans; transcriptomics; anti-aging; STRESS TOLERANCE; LIFE-SPAN; HEAT;
D O I
10.3390/antiox14010035
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study investigates the anti-aging effects of various concentrations of blueberry anthocyanins (BA) on the lifespan and health-related phenotypes of Caenorhabditis elegans. Blueberry anthocyanins were administered at concentrations of 50.0 mu g/mL, 200.0 mu g/mL, and 500.0 mu g/mL, and their effects on nematode lifespan, locomotion, pharyngeal pumping rate, and the accumulation of lipofuscin and reactive oxygen species (ROS) were examined. Transcriptomic analysis was conducted to explore the regulatory effects of BA on anti-aging molecular pathways and key genes in C. elegans. Results showed a significant, dose-dependent extension of lifespan, improvement in locomotion and pharyngeal pumping rate, and reduction in lipofuscin and ROS accumulation. Transcriptomic analysis revealed that BA activated anti-aging pathways such as FOXO, IIS, and PI3K/Akt, upregulating critical genes like daf-16. These findings highlight the potential of blueberry anthocyanins as promising anti-aging agents through multiple physiological and molecular mechanisms.
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页数:20
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