Anti-aging effect of recombinant Morchella eximia immunomodulatory protein on Caenorhabditis elegans

被引：0
作者：
Li, Jinyan [1 ]
Zhang, Jialan [2 ]
Gu, Tong [1 ]
Li, Li [1 ,3 ]
Gao, Mengxiang [1 ,3 ]
机构：
[1] Yangtze Univ, Coll Life Sci, Jingzhou 434025, Hubei, Peoples R China
[2] Yangtze Univ, Coll Anim Sci & Technol, Jingzhou 434025, Hubei, Peoples R China
[3] Yangtze Univ, Inst Food Sci & Technol, Jingzhou 434025, Hubei, Peoples R China
来源：
JOURNAL OF FUNCTIONAL FOODS | 2025年 / 126卷
基金：
中国国家自然科学基金;
关键词：
Morchella eximia; Fungal immunomodulatory protein; Anti-aging; Caenorhabditis elegans; RESTRICTION; ACTIVATION; MECHANISMS; CYTOKINE; STRESS; ACID;
D O I：
10.1016/j.jff.2025.106709
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A fungal immunomodulatory protein (FIP) (MeIMP) was discovered in edible mushroom Morchella eximia. Bioinformatics analysis demonstrated that MeIMP possesses cerato-platanin domains. Phylogenetic analysis positioned MeIMP within a distinct branch of the FIP family. The MeIMP gene was cloned and expressed in the Rosetta (DE3) strain. The anti-aging effect of recombinant MeIMP (rMeIMP) was evaluated by several indicators of Caenorhabditis elegans (C. elegans). The results indicated that rMeIMP significantly prolonged the lifespan of C. elegans, enhanced their motility ability, and diminished lipofuscin accumulation, while not affecting reproductive ability. Under UV, high temperature, and hydrogen peroxide stress, rMeIMP also significantly prolonged survival times of C. elegans. Additionally, rMeIMP extended the lifespan of C. elegans by scavenging ROS to mitigate the detrimental effects of oxidative stress. The establishment of a prokaryotic expression system for MeIMP and the assessment of rMeIMP function provided valuable insights for the further development and utilization of MeIMP.
引用
收藏
页数：10
相关论文
共 55 条
  • [51] Zhang D.-P., Jing X.-R., Fan A.-W., Liu H., Nie Y., Xu Y., Active expression of membrane-bound l-amino acid deaminase from proteus mirabilis in recombinant Escherichia coli by fusion with maltose-binding protein for enhanced catalytic performance, Catalysts, 10, 2, (2020)
  • [52] Zhang P., Zhai Y., Cregg J., Ang K.K.-H., Arkin M., Kenyon C., Stress resistance screen in a human primary cell line identifies small molecules that affect aging pathways and extend Caenorhabditis elegans’ lifespan, G3: Genes, Genomes, Genetics, 10, 2, pp. 849-862, (2020)
  • [53] Zhang Q., Wu C., Sun Y., Li T., Fan G., Cytoprotective effect of morchella esculenta protein hydrolysate and its derivative against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress, Polish Journal Of Food And Nutrition Sciences, 69, 3, pp. 255-265, (2019)
  • [54] Zhu J., Jia Y., Wang C., Zhou W., Shu Y., Zhang K., Guo R., Lonicera japonica polysaccharides improve longevity and fitness of Caenorhabditis elegans by activating DAF-16, International Journal of Biological Macromolecules, 229, pp. 81-91, (2023)
  • [55] Zou S., Wu Q., Li Z., Zhang S., Dong L., Chen Y., Lin X., Lactiplantibacillus plantarum A72, a strain with antioxidant properties, obtained through ARTP mutagenesis, affects Caenorhabditis elegans anti-aging, Foods, 13, 6, (2024)
123456