Anti-aging effect of recombinant Morchella eximia immunomodulatory protein on Caenorhabditis elegans

被引：0
作者：
Li, Jinyan [1 ]
Zhang, Jialan [2 ]
Gu, Tong [1 ]
Li, Li [1 ,3 ]
Gao, Mengxiang [1 ,3 ]
机构：
[1] Yangtze Univ, Coll Life Sci, Jingzhou 434025, Hubei, Peoples R China
[2] Yangtze Univ, Coll Anim Sci & Technol, Jingzhou 434025, Hubei, Peoples R China
[3] Yangtze Univ, Inst Food Sci & Technol, Jingzhou 434025, Hubei, Peoples R China
来源：
JOURNAL OF FUNCTIONAL FOODS | 2025年 / 126卷
基金：
中国国家自然科学基金;
关键词：
Morchella eximia; Fungal immunomodulatory protein; Anti-aging; Caenorhabditis elegans; RESTRICTION; ACTIVATION; MECHANISMS; CYTOKINE; STRESS; ACID;
D O I：
10.1016/j.jff.2025.106709
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A fungal immunomodulatory protein (FIP) (MeIMP) was discovered in edible mushroom Morchella eximia. Bioinformatics analysis demonstrated that MeIMP possesses cerato-platanin domains. Phylogenetic analysis positioned MeIMP within a distinct branch of the FIP family. The MeIMP gene was cloned and expressed in the Rosetta (DE3) strain. The anti-aging effect of recombinant MeIMP (rMeIMP) was evaluated by several indicators of Caenorhabditis elegans (C. elegans). The results indicated that rMeIMP significantly prolonged the lifespan of C. elegans, enhanced their motility ability, and diminished lipofuscin accumulation, while not affecting reproductive ability. Under UV, high temperature, and hydrogen peroxide stress, rMeIMP also significantly prolonged survival times of C. elegans. Additionally, rMeIMP extended the lifespan of C. elegans by scavenging ROS to mitigate the detrimental effects of oxidative stress. The establishment of a prokaryotic expression system for MeIMP and the assessment of rMeIMP function provided valuable insights for the further development and utilization of MeIMP.
引用
收藏
页数：10
相关论文
共 55 条
  • [1] Abdelfattah M.A.O., Dmirieh M., Ben Bakrim W., Mouhtady O., Ghareeb M.A., Wink M., Sobeh M., Antioxidant and anti-aging effects of Warburgia salutaris bark aqueous extract: Evidences from in silico, in vitro and in vivo studies, Journal of Ethnopharmacology, 292, (2022)
  • [2] Chang H.-H., Yeh C.-H., Sheu F., A novel immunomodulatory protein from poria cocos induces toll-like receptor 4-dependent activation within mouse peritoneal macrophages, Journal of Agricultural and Food Chemistry, 57, 14, pp. 6129-6139, (2009)
  • [3] Chen H., Wang S., Zhou A., Miao J., Liu J., Benjakul S., A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegans, Journal of Functional Foods, 68, (2020)
  • [4] Chen S., Wang M., Veeraperumal S., Teng B., Li R., Qian Z., Cheong K.-L., Antioxidative and protective effect of morchella esculenta against dextran sulfate sodium-induced alterations in liver, Foods, 12, 5, (2023)
  • [5] Chen W.-Y., Chang C.-Y., Li J.-R., Wang J.-D., Wu C.-C., Kuan Y.-H., Chen C.-J., Anti-inflammatory and neuroprotective effects of fungal immunomodulatory protein involving microglial inhibition, International Journal of Molecular Sciences, 19, 11, (2018)
  • [6] Chen X., Feng X., Kong X., Wang H., Liu T., Tang L., Zhuang Z., Design, synthesis, and anti-aging effect evaluation of urolithin B and its amide derivatives on nematode Caenorhabditis elegans, Medicinal Chemistry Research, 32, 6, pp. 1087-1097, (2023)
  • [7] Chu P.-Y., Sun H.-L., Ko J.-L., Ku M.-S., Lin L.-J., Lee Y.-T., Lue K.-H., Oral fungal immunomodulatory protein-Flammulina velutipes has influence on pulmonary inflammatory process and potential treatment for allergic airway disease: A mouse model, Journal of Microbiology, Immunology and Infection, 50, 3, pp. 297-306, (2017)
  • [8] Cui X., Zhang B., Li Z., Li C., Li J., Zhuyeqing liquor promotes longevity through enhancing stress resistance via regulation of SKN-1 and HSF-1 transcription factors in Caenorhabditis elegans, Experimental Gerontology, 174, (2023)
  • [9] Du Y., Hashizume T., Kurita-Ochiai T., Yuzawa S., Abiko Y., Yamamoto M., Blanke S.R., Nasal immunization with a fusion protein consisting of the hemagglutinin a antigenic region and the maltose-binding protein elicits CD11c+CD8+dendritic cells for induced long-term protective immunity, Infection and Immunity, 79, 2, pp. 895-904, (2011)
  • [10] Ejike U.C., Chan C.J., Okechukwu P.N., Lim R.L.H., New advances and potentials of fungal immunomodulatory proteins for therapeutic purposes, Critical Reviews in Biotechnology, 40, 8, pp. 1172-1190, (2020)
123456