Recent perspective of synthesis and modification strategies of cellulose nanocrystals and cellulose nanofibrils and their beneficial impact in scaffold-based tissue engineering: A review

Outstanding properties of nanocellulose provide opportunities for novel applications in various fields, particularly tissue engineering. Despite of numerous useful characteristics of nanocellulose, its production methods suffer from the lack of control of morphology, high cost, and the use of organic solvents. On the other hand, hydrophilicity of nanocellulose is a significant challenge for its dispersion as a reinforcement in hydrophobic polymers matrix. Therefore, sustainable production methods and well-tuning interfacial characteristics of nanocellulose have been identified as critical steps in their development. This review article discusses the numerous preparation methods and surface modification strategies of cellulose nanocrystals (CNCs) and cellulose nanofibrils (CNFs) to help nanocellulose users obtain the appropriate material for their desired application. We also cover various polymer/nanocellulose scaffolds that are reported in the literature and investigate the effect of CNC and CNF on their mechanical, thermal and biological properties. Moreover, we provide several scientific figures and tables for a better understanding of the explored topics. Finally, we evaluate the opportunities and challenges of nanocellulose industrialization in the field of tissue engineering. Overall, this review guides researchers towards a deeper understanding of nanocellulose production processes, changing their properties using surface modification methods, and subsequently their performance in scaffold-based tissue engineering.