Long-Term Effects of Biochar Application on Soil Heterotrophic Respiration in a Warm-Temperate Oak Forest

Biochar application as a soil amendment is gaining attention as a stable, long-term carbon sequestration strategy for the mitigation of climate change. However, biochar applied to the soil may increase soil carbon efflux. This study aimed to determine the long-term (8 years) effects of biochar application to the forest floor on soil carbon effluxes (soil respiration [SR] and heterotrophic respiration [HR]) in a warm-temperate oak forest. Biochar was applied at the rate of 0, 5, or 10 Mg ha(-1) to 20 m x 20 m plots (n = 4). The SR and HR rates were determined using the closed chamber method and the trenching method. The annual SR tended to increase over 8 years following biochar application, whereas a significant increase in the annual HR (+31%-37%) was observed in the short term (<3 years). The increased HR likely included CO2 emissions from the decomposition of the labile fraction of biochar carbon and from the microbial decomposition of the original soil organic matter stimulated through changes in the soil physicochemical environment, such as soil moisture and pH. The results suggest that a short-term increase in HR should be considered in the evaluation of carbon sequestration in response to biochar addition to forest ecosystems.