Tissue Culture and Rapid Micropropagation for Quercus suber L.

Quercus suber L. (Q. suber) is an evergreen tree species known for producing high-quality cork. Traditional seed propagation of Q. suber has low viability and is time-consuming. Therefore, we used young stem segments of 2 similar to 3-year-old seedlings as explants, and optimized protocols for tissue culture and rapid micropropagation of Q. suber. The best disinfection method was 0.10% HgCl2 (v/v) for 5 min. 0.50 g center dot L-1 Poly Vinyl Pyrrolidone (PVP) is the best anti-browning agent with a significant reduction in browning by nearly 1.76-fold (58.89% -> 33.33%). Woody Plant Medium supplemented with micronutrients and vitamins from Murashige and Skoog Medium (WPMS) was found to be the most suitable for shoot formation. The optimal hormone ratio for development of shoots from axillary buds was 0.60 mg center dot L-1 6-benzyladenine (6-BA). Among the cytokinins tested, 0.50 mg center dot L-1 6-BA was the most suitable for development of shoots from axillary buds. In additon, the highest percentage of rooting explants (66.67%) and rooting number (3.03) was obtained on WPM basal medium supplemented 0.20 mg center dot L-1 IBA and 0.20 mg center dot L-1 NAA. In summary, we have established a set of protocols for tissue culture and rapid micropropagation of Q. suber. These findings lay the foundation for rapid micropropagation and genetic improvement.