Effects of Alexandrium pacificum Exposure on Exopalaemon carinicauda: Hepatopancreas Histology, Antioxidant Enzyme Activity, and Transcriptome Analysis

被引：0

作者：

Han, Wanyu ^{[1
]}

Cheng, Weitao ^{[1
]}

Fan, Menghao ^{[1
]}

Liu, Dexue ^{[1
]}

Cao, Yanrong ^{[1
]}

Mei, Xuao ^{[1
]}

Wan, Jiaxuan ^{[1
]}

Hu, Guangwei ^{[1
,2
,3
]}

Gao, Huan ^{[1
,2
,3
]}

Ji, Nanjing ^{[1
,2
]}

机构：

[1] Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Environm, Lianyungang 222005, Peoples R China

[2] Jiangsu Ocean Univ, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang 222005, Peoples R China

[3] Jiangsu Prov Infrastruct Conservat & Utilizat Agr, Nanjing 210014, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2025年 / 26卷 / 04期

关键词：

<italic>Exopalaemon carinicauda</italic>; <italic>Alexandrium pacificum</italic>; transcriptomic; hepatopancreas damage; harmful algal blooms; OXIDATIVE STRESS; GENE; METABOLISM; DIVERSITY; TOXICITY; NITRITE; BLOOMS; ROLES;

D O I：

10.3390/ijms26041605

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alexandrium pacificum, a dinoflagellate known for causing harmful algal blooms (HABs), has garnered significant attention due to its potential toxicity to marine ecosystems, fisheries, and human health. However, the effects of this toxin-producing alga on shrimp are not yet comprehensively understood. This study aimed to assess the hepatopancreas damage induced by A. pacificum in the economically important shrimp species E. carinicauda and to elucidate the underlying molecular mechanisms through histology, antioxidant enzyme activity, and transcriptome analysis. The shrimp were assigned to either a control group or an exposed group, with the latter involving exposure to A. pacificum at a concentration of 1.0 x 104 cells/mL for 7 days. A histological analysis subsequently revealed pathological changes in the hepatopancreas tissue of the exposed group, including lumen expansion and the separation of the basement membrane from epithelial cells, while antioxidant enzyme activity assays demonstrated that exposure to A. pacificum weakened the antioxidant defense system, as evidenced by the reduced activities of catalase, superoxide dismutase, and glutathione, along with increased malondialdehyde levels. Transcriptome analysis further identified 663 significantly upregulated genes and 1735 significantly downregulated ones in the exposed group, with these differentially expressed genes being primarily associated with pathways such as protein processing in the endoplasmic reticulum, mitophagy, glycolysis/gluconeogenesis, sphingolipid metabolism, and glycerophospholipid metabolism. This study provides novel insights into the toxicological effects of A. pacificum on aquatic organisms and enhances the current understanding of the ecotoxicological risks posed by HABs.

引用

页数：16

共 70 条

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