Intelligent Point-of-Care Microchip Based on Ternary Hybrids: Incorporating AIEgens and Nanozymes into Metal-Organic Frameworks

被引：0

作者：

Feng, Niu ^{[1
,2
]}

Huang, Xiaolin ^{[3
]}

Zhao, Yongkun ^{[1
,2
]}

Wang, Qinyu ^{[4
]}

Peng, Xuewen ^{[1
,2
]}

Dong, Yiming ^{[1
,2
]}

Xue, Li ^{[5
]}

Tang, Ben Zhong ^{[6
]}

Chen, Yiping ^{[1
,2
]}

机构：

[1] Dalian Polytech Univ, State Key Lab Marine Food Proc & Safety Control, Dalian 116034, Liaoning, Peoples R China

[2] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China

[3] Nanchang Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Resources, Nanchang 330047, Jiangxi, Peoples R China

[4] South China Univ Technol, Sch Future Technol, Guangzhou 510641, Guangdong, Peoples R China

[5] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China

[6] Chinese Univ Hong Kong, Shenzhen Inst Aggregate Sci & Technol, Sch Sci & Engn, Shenzhen 518172, Guangdong, Peoples R China

来源：

ADVANCED FUNCTIONAL MATERIALS | 2024年

基金：

中国国家自然科学基金;

关键词：

aggregation-induced emission; biomarkers; hybrid nanoprobes; metal-organic frameworks; nanozymes; point-of-care testing; ON-DEMAND; MOF; LUMINESCENCE; MORPHOLOGIES;

D O I：

10.1002/adfm.202410900

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The risk of disease highlights the necessity of point-of-care testing. Here, a ternary hybrid (MAP) combining metal-organic frameworks (MOFs), aggregate-induced emission luminogens, and Pt nanoparticles is introduced. MAP demonstrates a greater fluorescence quantum yield (62.1%) and greater anti-photobleaching ability than conventional fluorescent MOFs and organic fluorophores, as well as a 2214-fold and 16-fold increase in catalytic capacity over those of horseradish peroxidase and Pt nanoparticles, respectively. The MAP exhibited a slight attenuation after 180 days at room temperature in signal intensity. Polydopamine-coated polystyrene microsphere-enabled microchips are economical, user-friendly, and portable. The combination of MAP and microchips allows for determination using the fluorescence/colorimetric mode in different scenarios. Compared with the enzyme-linked immunosorbent assay, the fluorescence mode exhibited a 41.9-fold increase in sensitivity, a 94.2% decrease in time, and an 80.7% decrease in cost. The accuracy of clinical samples reached 100%, and the specificity is 96.2%. Chloramphenicol and enrofloxacin serve as small molecule targets, demonstrating the versatility of this strategy.

引用

页数：15

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