Exploring quercetin's protective potential against the pro-inflammatory effects of silver nanoparticles in C57BL/6J mice

The increasing prevalence of silver nanoparticles (AgNP) in various applications has sparked concerns about their potential adverse effects on human health. Hence, it is crucial to devise strategies to minimize their detrimental effects. Quercetin, a naturally occurring flavonoid present in human diet is known for its broad biological effects, including anti-inflammatory properties. Considering this, quercetin could serve as a promising strategy to protect the body against the harmful effects of AgNP. Thus, this study aimed to evaluate the potential protective role of quercetin against the deleterious effects induced by 5 nm polyvinylpyrrolidone (PVP)-AgNP in C57BL/6J mice. Using a novel administration technology (HaPILLness), mice were given a daily oral dose of AgNP at 1 mg/kg body weight (bw) or 10 mg/kg bw for 14 days, combined with daily IP injections of quercetin at 1 mg/kg bw. Our findings demonstrate that quercetin effectively attenuated the AgNP-induced intestinal inflammatory response, as demonstrated by reduced histological vascular and cellular alterations, along with a notable decrease in cytokine production, attributed to the inhibition of the nuclear factor (NF)-& kcy;B inflammatory pathway. Quercetin's protective effects extended to the liver and lungs, by reversing changes in the inflammatory and antioxidant markers cluster of differentiation (CD)4, superoxide dismutase 1 (SOD1) and catalase.