Research Progress and Application of Interfacing of Supercritical Fluid Chromatography and Mass Spectrometry

In the past few decades, supercritical fluid chromatography (SFC) as a supplement to liquid chromatography (LC) separation technology has attracted people ' s interest, especially in the combination of SFC and mass spectrometry (MS), which has shown important application prospects in metabolomics, lipidomics, and other fields. Compared to the interface of LC-MS, the interface of SFC-MS presents some unique challenges that require special solutions to be designed. This article categorizes and summarizes the existing interfaces used for SFC-MS, focuses on the impact of different interface designs on detection performance, provides the applicable characteristics of different types of interfaces, and finally briefly introduces the application progress of SFC-MS in different fields.