In the current investigation, Cu/Ag/Ru trimetallic nanoparticles (TNPs) were produced using Andrographispaniculata, a medicinal herb known for its antibacterial, anticancer, antifungal, antiviral properties, and extensive therapeutic uses. Recently, nanotechnology has become essential for the progress of research and addressing environmental pollution issues.These trimetallic or polymetallic nanoparticles has been synthesized and characterized further.The presence of TNPs was confirmed through UV, FTIR, SEM, and EDX analyses. UV studies indicated that the TNPs exhibited absorption peaks within the range of 200 to 400 nm. The various phytochemical constituents such as alkaloids, terpenoids, aldehydes, ketones, and phenols present in the plant were identified by FTIR analysis. The size, morphology, elemental composition, crystallinity, and three-dimensional structure of the TNPs were verified using SEM, EDX, XRD, and AFM techniques.The dimensions of theTNPs ranged from 20 to 60 nm.TheTNPs demonstrated remarkable results in both antibacterial and photocatalytic activities, particularly in dye degradation. The different bacterial strains were utilized effectively for anticancer studies. To reduce the use of harmful substances that can impact the environment, the TNPs were synthesized biologically, making them eco-friendly, cost-effective, and devoid of harmful byproducts in the synthesis process.