Mechanical and In vitro evaluation of cell structures for bone tissue engineering

Additive stereolithography manufacturing has been one of the medical industry's 3D printing techniques and the most successful applications. In the present project, cellular scaffolds with variable porosities (66 %, 74 %, and 82 %) of two geometries (Gyroid and Kelvin) were designed and fabricated chemically, mechanically, and structurally. The samples were evaluated in vitro acellular and with the cell primary dermal fibroblasts line. The results obtained from Young's Modulus of the Gyroid and Kelvin cell structures were 0.499 MPa and 0.312 MPa in the samples with 66 % and 74 % porosities, respectively. On the other hand, the maximum stress results for the Gyroid sample with 6 x 6 unit cells was 7527 MPa, and for the 66 % porosity Kelvin scaffold with 4 x 4 unit cells, it was 7261 MPa. The samples presented a favorable degradability and bioactivity after 7 days. The results suggest that resin may be an excellent alternative for manufacturing medical devices for bone tissue using the SLA technique, with a short-term temporality tailored to each patient who requires it.