Decreased histone expression in chronic rhinosinusitis with nasal polyps

被引：0
|
作者：
Zhao, Yanming [1 ]
Zhang, Nan [2 ]
Novo, Claudina Perez [2 ]
Wang, Yang [3 ]
Zhang, Luo [1 ,3 ,4 ]
机构：
[1] Capital Med Univ, Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, Beijing, Peoples R China
[2] Ghent Univ Hosp, Upper Airway Res Lab URL, Dept Otorhinolaryngol, Ghent, Belgium
[3] Beijing Inst Otolaryngol, Beijing Key Lab Nasal Dis, Beijing, Peoples R China
[4] Capital Med Univ, Beijing Tongren Hosp, Dept Allergy, Beijing, Peoples R China
来源：
ASIA PACIFIC ALLERGY | 2024年 / 14卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Apoptosis; histones; microarray analysis; nasal polyps; sinusitis; EXTRACELLULAR HISTONES; APOPTOSIS; PHOSPHORYLATION; INVOLVEMENT; H2B;
D O I：
10.5415/apallergy.0000000000000140
中图分类号：
R392 [医学免疫学];
学科分类号：
100102 ;
摘要：
Background: Histones have been associated with human diseases. However, the implication of extranuclear histone proteins and their potential mechanism in the pathophysiology of chronic rhinosinusitis (CRS) have not been thoroughly investigated. This study was designed to evaluate the role of histones in patients with CRS by comparing histone expression between patients and controls. Methods: Nasal polyp (NP) tissues were obtained, and their comprehensive gene expression profiles were investigated by microarray analysis. Differences in expression were verified by reverse transcriptase polymerase chain reaction and immunohistochemical staining. Cell culture and flow cytometry were used to evaluate the role of histones in the pathogenesis of polyps. Results: Significant differences in the microarray analysis were observed between the patient and control groups (P < 0.01). It was found by flow cytometry that the histone (H2BK) can promote cell apoptosis in NPs. Conclusion: Our results indicate that reduced expression of H2BK may contribute to the imbalance process of cell proliferation and apoptosis in CRS with NP.
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收藏
页码：70 / 76
页数：7
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